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Mouse Positron Emission Tomography Study of the Biodistribution of Gold Nanoparticles with Different Surface Coatings Using Embedded Copper-64

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  • Anders F Frellsen
  • Anders E Hansen
  • Rasmus I Jølck
  • Paul J Kempen
  • Gregory W Severin
  • Palle H Rasmussen
  • Andreas Kjær
  • Andreas T I Jensen
  • Thomas L Andresen
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By taking advantage of the ability of (64)Cu to bind nonspecifically to gold surfaces, we have developed a methodology to embed this radionuclide inside gold nanoparticles (AuNPs). (64)Cu enables the in vivo imaging of AuNPs by positron emission tomography (PET). AuNPs have a multitude of uses within health technology and are useful tools for general nanoparticle research. (64)Cu-AuNPs were prepared by incubating AuNP seeds with (64)Cu(2+), followed by the entrapment of the radionuclide by grafting on a second layer of gold. This resulted in radiolabeling efficiencies of 53 ± 6%. The radiolabel showed excellent stability when incubated with EDTA for 2 days (95% radioactivity retention) and showed no loss of (64)Cu when incubated with 50% mouse serum for 2 days. The methodology was chelator-free, removing traditional concerns over chelator instability and altered AuNP properties due to surface modification. Radiolabeled (64)Cu-AuNP cores were prepared in biomedically relevant sizes of 20-30 nm and used to investigate the in vivo stability of three different AuNP coatings by PET imaging in a murine xenograft tumor model. We found the longest plasma half-life (T1/2 about 9 h) and tumor accumulation (3.9%ID/g) to result from a polyethylene glycol coating, while faster elimination from the bloodstream was observed with both a Tween 20-stabilized coating and a zwitterionic coating based on a mixture of sulfonic acids and quaternary amines. In the in vivo model, the (64)Cu was observed to closely follow the AuNPs for each coating, again attributed to the excellent stability of the radiolabel.

Original languageEnglish
JournalACS Nano
Volume10
Issue number11
Pages (from-to)9887-9898
Number of pages12
ISSN1936-0851
DOIs
Publication statusPublished - 22 Nov 2016

ID: 49873049