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Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment

Research output: Contribution to journalJournal articleResearchpeer-review

  • Felix Elortza
  • Shabaz Mohammed
  • Jakob Bunkenborg
  • Leonard J Foster
  • Thomas S Nühse
  • Urs Brodbeck
  • Scott C Peck
  • Ole N Jensen
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Plasma membrane proteins are displayed through diverse mechanisms, including anchoring in the extracellular leaflet via glycosylphosphatidylinositol (GPI) molecules. GPI-anchored membrane proteins (GPI-APs) are a functionally and structurally diverse protein family, and their importance is well-recognized as they are candidate cell surface biomarker molecules with potential diagnostic and therapeutic applications in molecular medicine. GPI-APs have also attracted interest in plant biotechnology because of their role in root development and cell remodeling. Using a shave-and-conquer concept, we demonstrate that phospholipase D (PLD) treatment of human and plant plasma membrane fractions leads to the release of GPI-anchored proteins that were identified and characterized by capillary liquid chromatography and tandem mass spectrometry. In contrast to phospholipase C, the PLD enzyme is not affected by structural heterogeneity of the GPI moiety, making PLD a generally useful reagent for proteomic investigations of GPI-anchored proteins in a variety of cells, tissues, and organisms. A total of 11 human GPI-APs and 35 Arabidopsis thaliana GPI-APs were identified, representing a significant addition to the number of experimentally detected GPI-APs in both species. Computational GPI-AP sequence analysis tools were investigated for the characterization of the identified GPI-APs, and these demonstrated that there is some discrepancy in their efficiency in classification of GPI-APs and the exact assignment of omega-sites. This study highlights the efficiency of an integrative proteomics approach that combines experimental and computational methods to provide the selectivity, specificity, and sensitivity required for characterization of post-translationally modified membrane proteins.
Original languageEnglish
JournalJournal of Proteome Research
Volume5
Issue number4
Pages (from-to)935-43
Number of pages9
ISSN1535-3893
DOIs
Publication statusPublished - 2006
Externally publishedYes

    Research areas

  • Amino Acid Sequence, Animals, Arabidopsis, Cattle, Cell Fractionation, Cell Membrane, Chromatography, Liquid, Databases, Factual, Electrophoresis, Capillary, Glycosylphosphatidylinositols, HeLa Cells, Humans, Hydrophobic and Hydrophilic Interactions, Membrane Microdomains, Membrane Proteins, Molecular Sequence Data, Phospholipase D, Protein Processing, Post-Translational, Protein Structure, Tertiary, Proteome, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Trypsin

ID: 42360872