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Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo

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Kapishnikov, S, Staalsø, T, Yang, Y, Lee, J, Pérez-Berná, AJ, Pereiro, E, Yang, Y, Werner, S, Guttmann, P, Leiserowitz, L & Als-Nielsen, J 2019, 'Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo' Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 46, pp. 22946-22952. https://doi.org/10.1073/pnas.1910123116

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Kapishnikov, Sergey ; Staalsø, Trine ; Yang, Yang ; Lee, Jiwoong ; Pérez-Berná, Ana J ; Pereiro, Eva ; Yang, Yang ; Werner, Stephan ; Guttmann, Peter ; Leiserowitz, Leslie ; Als-Nielsen, Jens. / Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo. In: Proceedings of the National Academy of Sciences of the United States of America. 2019 ; Vol. 116, No. 46. pp. 22946-22952.

Bibtex

@article{64facaed775a4673b5df7d343475dac8,
title = "Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo",
abstract = "The most widely used antimalarial drugs belong to the quinoline family. Their mode of action has not been characterized at the molecular level in vivo. We report the in vivo mode of action of a bromo analog of the drug chloroquine in rapidly frozen Plasmodium falciparum-infected red blood cells. The Plasmodium parasite digests hemoglobin, liberating the heme as a byproduct, toxic to the parasite. It is detoxified by crystallization into inert hemozoin within the parasitic digestive vacuole. By mapping such infected red blood cells with nondestructive X-ray microscopy, we observe that bromoquine caps hemozoin crystals. The measured crystal surface coverage is sufficient to inhibit further hemozoin crystal growth, thereby sabotaging heme detoxification. Moreover, we find that bromoquine accumulates in the digestive vacuole, reaching submillimolar concentration, 1,000-fold more than that of the drug in the culture medium. Such a dramatic increase in bromoquine concentration enhances the drug's efficiency in depriving heme from docking onto the hemozoin crystal surface. Based on direct observation of bromoquine distribution in the digestive vacuole and at its membrane surface, we deduce that the excess bromoquine forms a complex with the remaining heme deprived from crystallization. This complex is driven toward the digestive vacuole membrane, increasing the chances of membrane puncture and spillage of heme into the interior of the parasite.",
author = "Sergey Kapishnikov and Trine Staals{\o} and Yang Yang and Jiwoong Lee and P{\'e}rez-Bern{\'a}, {Ana J} and Eva Pereiro and Yang Yang and Stephan Werner and Peter Guttmann and Leslie Leiserowitz and Jens Als-Nielsen",
note = "Copyright {\circledC} 2019 the Author(s). Published by PNAS.",
year = "2019",
month = "11",
day = "12",
doi = "10.1073/pnas.1910123116",
language = "English",
volume = "116",
pages = "22946--22952",
journal = "National Academy of Sciences. Proceedings",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "46",

}

RIS

TY - JOUR

T1 - Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo

AU - Kapishnikov, Sergey

AU - Staalsø, Trine

AU - Yang, Yang

AU - Lee, Jiwoong

AU - Pérez-Berná, Ana J

AU - Pereiro, Eva

AU - Yang, Yang

AU - Werner, Stephan

AU - Guttmann, Peter

AU - Leiserowitz, Leslie

AU - Als-Nielsen, Jens

N1 - Copyright © 2019 the Author(s). Published by PNAS.

PY - 2019/11/12

Y1 - 2019/11/12

N2 - The most widely used antimalarial drugs belong to the quinoline family. Their mode of action has not been characterized at the molecular level in vivo. We report the in vivo mode of action of a bromo analog of the drug chloroquine in rapidly frozen Plasmodium falciparum-infected red blood cells. The Plasmodium parasite digests hemoglobin, liberating the heme as a byproduct, toxic to the parasite. It is detoxified by crystallization into inert hemozoin within the parasitic digestive vacuole. By mapping such infected red blood cells with nondestructive X-ray microscopy, we observe that bromoquine caps hemozoin crystals. The measured crystal surface coverage is sufficient to inhibit further hemozoin crystal growth, thereby sabotaging heme detoxification. Moreover, we find that bromoquine accumulates in the digestive vacuole, reaching submillimolar concentration, 1,000-fold more than that of the drug in the culture medium. Such a dramatic increase in bromoquine concentration enhances the drug's efficiency in depriving heme from docking onto the hemozoin crystal surface. Based on direct observation of bromoquine distribution in the digestive vacuole and at its membrane surface, we deduce that the excess bromoquine forms a complex with the remaining heme deprived from crystallization. This complex is driven toward the digestive vacuole membrane, increasing the chances of membrane puncture and spillage of heme into the interior of the parasite.

AB - The most widely used antimalarial drugs belong to the quinoline family. Their mode of action has not been characterized at the molecular level in vivo. We report the in vivo mode of action of a bromo analog of the drug chloroquine in rapidly frozen Plasmodium falciparum-infected red blood cells. The Plasmodium parasite digests hemoglobin, liberating the heme as a byproduct, toxic to the parasite. It is detoxified by crystallization into inert hemozoin within the parasitic digestive vacuole. By mapping such infected red blood cells with nondestructive X-ray microscopy, we observe that bromoquine caps hemozoin crystals. The measured crystal surface coverage is sufficient to inhibit further hemozoin crystal growth, thereby sabotaging heme detoxification. Moreover, we find that bromoquine accumulates in the digestive vacuole, reaching submillimolar concentration, 1,000-fold more than that of the drug in the culture medium. Such a dramatic increase in bromoquine concentration enhances the drug's efficiency in depriving heme from docking onto the hemozoin crystal surface. Based on direct observation of bromoquine distribution in the digestive vacuole and at its membrane surface, we deduce that the excess bromoquine forms a complex with the remaining heme deprived from crystallization. This complex is driven toward the digestive vacuole membrane, increasing the chances of membrane puncture and spillage of heme into the interior of the parasite.

U2 - 10.1073/pnas.1910123116

DO - 10.1073/pnas.1910123116

M3 - Journal article

VL - 116

SP - 22946

EP - 22952

JO - National Academy of Sciences. Proceedings

JF - National Academy of Sciences. Proceedings

SN - 0027-8424

IS - 46

ER -

ID: 58999349