TY - JOUR

T1 - Mining the granule proteome

T2 - a potential source of endocrine biomarkers

AU - Albrethsen, Jakob

AU - Goetze, Jens P

AU - Johnsen, Anders H

PY - 2015

Y1 - 2015

N2 - Proteomics of secretory granules is an emerging strategy for identifying secreted proteins, including potentially novel candidate biomarkers and peptide hormones. In addition, proteomics can provide information about the abundance, localization and structure (post-translational modification) of granule proteins and peptides. Analytical strategies within this research line include so-called 'subtractive proteomics', 'peptidomics' and granule purification by the use of multiple gradient centrifugations. Here we review the literature, and describe the challenges and opportunities in proteomics of secretory granules.

AB - Proteomics of secretory granules is an emerging strategy for identifying secreted proteins, including potentially novel candidate biomarkers and peptide hormones. In addition, proteomics can provide information about the abundance, localization and structure (post-translational modification) of granule proteins and peptides. Analytical strategies within this research line include so-called 'subtractive proteomics', 'peptidomics' and granule purification by the use of multiple gradient centrifugations. Here we review the literature, and describe the challenges and opportunities in proteomics of secretory granules.

KW - Biomarkers

KW - Endocrine Cells

KW - Humans

KW - Peptides

KW - Proteome

KW - Proteomics

KW - Secretory Vesicles

U2 - 10.2217/bmm.14.107

DO - 10.2217/bmm.14.107

M3 - Journal article

VL - 9

SP - 259

EP - 265

JO - Biomarker Research

JF - Biomarker Research

SN - 2050-7771

IS - 3

ER -