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Microcrack surface density in the human otic capsule: An unbiased stereological quantification

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Bone signaling in middle ear development: a genome-wide differential expression analysis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The viability of perilabyrinthine osteocytes: a quantitative study using bulk-stained undecalcified human temporal bones

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The three-dimensional arrangement of the myocytes aggregated together within the mammalian ventricular myocardium

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Macrophage-like cells in the muscularis externa of mouse small intestine

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Identification of Cellular Voids in the Human Otic Capsule

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. OpenEar Image Data Enables Case Variation in High Fidelity Virtual Reality Ear Surgery

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Distribution of microcrack surface density in the human otic capsule

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Cellular Patterns and Irregularities in human Perilabyrinthine Bone

    Research output: Book/ReportPh.D. thesis

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Bone is continuously remodeled to repair and strengthen degenerative bone with accumulating dead osteocytes and microfractures. Inner ear osteoprotegerin (OPG)-mediated inhibition of otic capsular bone remodeling causes excessive perilabyrinthine bone degeneration. Consequently, microcracks accumulate around the inner ear. Microcracks cause osteocyte apoptosis and may disrupt the canalicular network connecting osteocytes. Despite their linear microscopic appearance, microcracks are three-dimensional disruption planes and represent surface areas inside a tissue space. With an elevated microcrack burden the number of disconnected osteocytes is expected to increase. This may prove relevant to ongoing research in otic focal pathologies like otosclerosis. Therefore, an unbiased quantification of the microcrack surface density (mm2 /mm3 ) in the human otic capsule is essential. In this study unbiased stereology was applied to undecalcified bulk stained human temporal bones to demonstrate its feasibility in describing the three-dimensional reality behind two dimensional observations of microcracks. A total of 28 human temporal bones and five ribs were bulk stained in basic fuchsin, serially sectioned and hand-ground to a thickness of 80-120 μm. Both horizontal and vertical sections were produced and compared. This study showed that surface density of microcracks was significantly higher around the inner ear compared to ribs. Furthermore, no significant difference in microcrack surface density between horizontal and vertical sections in the temporal bone was demonstrated.

Original languageEnglish
JournalAnatomical record (Hoboken, N.J. : 2007)
Volume304
Issue number5
Pages (from-to)961-967
Number of pages7
ISSN1932-8486
DOIs
Publication statusPublished - May 2021

    Research areas

  • microcrack, microfracture, OPG, otosclerosis, surface density

ID: 61231258