Abstract
In a genetically engineered V79 cell line (XEMd-MZ) expressing rat cytochrome P4501A2 the activity of phenacetin-O-deethylase was determined and compared with freshly isolated rat hepatocytes. In the V79 cells the apparent Km was 0.99 microM (N = 4), compared to the high affinity Km (0.23 microM, N = 4) found in freshly isolated rat hepatocytes, where as the Vmax found in the XEMd-MZ cells (14.90 pmol/min/10(6) cells) corresponded to the Vmax for the high affinity site of the hepatocytes (18.2 pmol/min/10(-6) cells). The metabolic capacity of phenacetin was quantitatively comparable to that of freshly isolated hepatocytes on a cell to cell basis.
| Original language | English |
|---|---|
| Journal | Biochemical Pharmacology |
| Volume | 45 |
| Issue number | 5 |
| Pages (from-to) | 1171-3 |
| Number of pages | 3 |
| ISSN | 0006-2952 |
| DOIs | |
| Publication status | Published - 9 Mar 1993 |
Keywords
- Animals
- Cells, Cultured
- Cricetinae
- Cricetulus
- Cytochrome P-450 CYP1A2
- Cytochrome P-450 Enzyme System/genetics
- Liver/cytology
- Male
- Oxidoreductases/genetics
- Phenacetin/metabolism
- Rats
- Rats, Wistar
- Transfection
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