Metabolic footprint of Lactobacillus acidophilus NCFM at different pH

Karolina Sulek; Henrik Lauritz Frandsen; Jørn Smedsgaard; Thomas Hjort Skov; Andrea Wilcks; Tine Rask Licht

doi:10.1007/s11306-011-0305-4

Metabolic footprint of Lactobacillus acidophilus NCFM at different pH

Karolina Sulek^*, Henrik Lauritz Frandsen, Jørn Smedsgaard, Thomas Hjort Skov, Andrea Wilcks, Tine Rask Licht

^*Corresponding author for this work

12 Citations (Scopus)

Abstract

Lactobacillus acidophilus NCFM is a well known microorganism from the genomic and probiotic point of view. In order to analyze the potential interactions of NCFM with the surrounding environment, in vitro tests with the metabolic footprinting approach were performed. It was found that NCFM increased the concentration of lactic acid, succinic acid, adenine and arginine in the medium. The metabolism of NCFM did not change significantly between pH 5 and 7, suggesting that other environmental factors than pH might have bigger impact on its colonization throughout the gastrointestinal tract.

Original language	English
Journal	Metabolomics
Volume	8
Issue number	2
Pages (from-to)	244-252
Number of pages	9
ISSN	1573-3882
DOIs	https://doi.org/10.1007/s11306-011-0305-4
Publication status	Published - Apr 2012
Externally published	Yes

Keywords

GIT
Lactobacillus acidophilus NCFM
LCMS
Metabolite footprint
Metabolomics
Probiotics

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10.1007/s11306-011-0305-4

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title = "Metabolic footprint of Lactobacillus acidophilus NCFM at different pH",

abstract = "Lactobacillus acidophilus NCFM is a well known microorganism from the genomic and probiotic point of view. In order to analyze the potential interactions of NCFM with the surrounding environment, in vitro tests with the metabolic footprinting approach were performed. It was found that NCFM increased the concentration of lactic acid, succinic acid, adenine and arginine in the medium. The metabolism of NCFM did not change significantly between pH 5 and 7, suggesting that other environmental factors than pH might have bigger impact on its colonization throughout the gastrointestinal tract.",

keywords = "GIT, Lactobacillus acidophilus NCFM, LCMS, Metabolite footprint, Metabolomics, Probiotics",

author = "Karolina Sulek and Frandsen, \{Henrik Lauritz\} and J{\o}rn Smedsgaard and Skov, \{Thomas Hjort\} and Andrea Wilcks and Licht, \{Tine Rask\}",

note = "Funding Information: Acknowledgments This work was supported by a grant from the {\O}resund Food (co-financed by The European Union—The European Regional Development Fund and V{\ae}kstforum Hovedstaden; grant no. 09198-02 {\textquoteleft}{\textquoteleft}Bacterial Impact on the Gut Metabolome{\textquoteright}{\textquoteright}) and by the Danish Strategic Research Council{\textquoteright}s Committee on Food and Health (F{\o}su; project no. 2101-06-0067 {\textquoteleft}{\textquoteleft}Biological Production of Dietary Fibres and Prebiotics{\textquoteright}{\textquoteright}). Authors would like to thank Naseer Mahmoud Shukri and Tina Beck Hansen for their contribution to this work as well as Kate Vina Vibefeldt and Bodil Madsen for excellent technical support.",

year = "2012",

month = apr,

doi = "10.1007/s11306-011-0305-4",

language = "English",

volume = "8",

pages = "244--252",

journal = "Metabolomics",

issn = "1573-3882",

publisher = "Springer",

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TY - JOUR

T1 - Metabolic footprint of Lactobacillus acidophilus NCFM at different pH

AU - Sulek, Karolina

AU - Frandsen, Henrik Lauritz

AU - Smedsgaard, Jørn

AU - Skov, Thomas Hjort

AU - Wilcks, Andrea

AU - Licht, Tine Rask

N1 - Funding Information: Acknowledgments This work was supported by a grant from the Øresund Food (co-financed by The European Union—The European Regional Development Fund and Vækstforum Hovedstaden; grant no. 09198-02 ‘‘Bacterial Impact on the Gut Metabolome’’) and by the Danish Strategic Research Council’s Committee on Food and Health (Føsu; project no. 2101-06-0067 ‘‘Biological Production of Dietary Fibres and Prebiotics’’). Authors would like to thank Naseer Mahmoud Shukri and Tina Beck Hansen for their contribution to this work as well as Kate Vina Vibefeldt and Bodil Madsen for excellent technical support.

PY - 2012/4

Y1 - 2012/4

N2 - Lactobacillus acidophilus NCFM is a well known microorganism from the genomic and probiotic point of view. In order to analyze the potential interactions of NCFM with the surrounding environment, in vitro tests with the metabolic footprinting approach were performed. It was found that NCFM increased the concentration of lactic acid, succinic acid, adenine and arginine in the medium. The metabolism of NCFM did not change significantly between pH 5 and 7, suggesting that other environmental factors than pH might have bigger impact on its colonization throughout the gastrointestinal tract.

AB - Lactobacillus acidophilus NCFM is a well known microorganism from the genomic and probiotic point of view. In order to analyze the potential interactions of NCFM with the surrounding environment, in vitro tests with the metabolic footprinting approach were performed. It was found that NCFM increased the concentration of lactic acid, succinic acid, adenine and arginine in the medium. The metabolism of NCFM did not change significantly between pH 5 and 7, suggesting that other environmental factors than pH might have bigger impact on its colonization throughout the gastrointestinal tract.

KW - GIT

KW - Lactobacillus acidophilus NCFM

KW - LCMS

KW - Metabolite footprint

KW - Metabolomics

KW - Probiotics

UR - https://www.scopus.com/pages/publications/84857788833

U2 - 10.1007/s11306-011-0305-4

DO - 10.1007/s11306-011-0305-4

M3 - Journal article

AN - SCOPUS:84857788833

SN - 1573-3882

VL - 8

SP - 244

EP - 252

JO - Metabolomics

JF - Metabolomics

IS - 2

ER -

Metabolic footprint of Lactobacillus acidophilus NCFM at different pH

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Keywords

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