Measuring histamine and cytokine release from basophils and mast cells

Bettina M Jensen; Sidsel Falkencrone; Per S Skov

doi:10.1007/978-1-4939-1173-8_10

Measuring histamine and cytokine release from basophils and mast cells

Bettina M Jensen, Sidsel Falkencrone, Per S Skov

Dermatology and Allergy

12 Citations (Scopus)

Abstract

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

Original language	English
Journal	Methods in molecular biology
Volume	1192
Pages (from-to)	135-45
Number of pages	11
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-4939-1173-8_10
Publication status	Published - 2014

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abstract = "Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.",

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AU - Falkencrone, Sidsel

AU - Skov, Per S

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N2 - Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

AB - Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

UR - https://www.scopus.com/pages/publications/84927704309

U2 - 10.1007/978-1-4939-1173-8_10

DO - 10.1007/978-1-4939-1173-8_10

M3 - Journal article

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SN - 1064-3745

VL - 1192

SP - 135

EP - 145

JO - Methods in molecular biology

JF - Methods in molecular biology

ER -

Measuring histamine and cytokine release from basophils and mast cells

Abstract

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