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Measurement of the total proANP product in mammals by processing independent analysis

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  1. An IgY-based immunoassay to evaluate the biomarker potential of the Tannerella forsythia virulence factor karilysin in human saliva

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  2. The use of synthetic peptides for detection of anti-citrullinated protein antibodies in rheumatoid arthritis

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  3. A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product

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  4. Efficient evaluation of humoral immune responses by the use of serum pools

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  1. Oral D/L-3-Hydroxybutyrate stimulates cholecystokinin and insulin secretion and slows gastric emptying in healthy males

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  2. Bilio-enteric flow and plasma concentrations of bile acids after gastric bypass and sleeve gastrectomy

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  3. Effect of insulin on natriuretic peptide gene expression in porcine heart

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The cellular processing of natriuretic propeptides is attenuated in heart disease, resulting in release of a mixture of unprocessed precursor, partially processed fragments, and the bioactive hormone. Here, we report a species-independent method for quantification of pro-atrial natriuretic peptide (proANP) and its products irrespective of variable post-translational processing. The processing-independent assay (PIA) was developed raising mono-specific antibodies against the C-terminus of sequence 1-16 in proANP. The assay procedure included plasma extraction followed by tryptic cleavage, which releases the assay epitope from the N-terminal region. The PIA was tested in elderly patients with symptoms of heart failure (n=450), in pigs with acute myocardial infarction (n=21), and in normal dogs and dogs with heart failure (n=77). The epitope specificity permitted reliable measurement in man, dog, cat and pig. In human plasma, the PIA correlated well with an established proANP analysis (r=0.86, P
Original languageEnglish
JournalJournal of Immunological Methods
Volume370
Issue number1-2
Pages (from-to)104-10
Number of pages7
ISSN0022-1759
DOIs
Publication statusPublished - 2011

    Research areas

  • Amino Acid Sequence, Animals, Atrial Natriuretic Factor, Heart Failure, Humans, Molecular Sequence Data, Sequence Alignment

ID: 33124852