Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Gram-positive, catalase-negative cocci not belonging to the Streptococcus or Enterococcus genus and benefits of database extension

Jens Jørgen Christensen, Rimtas Dargis, Monja Hammer, Ulrik Stenz Justesen, Xiaohui C Nielsen, Michael Kemp, Danish MALDI-TOF MS Study Group, Michael Tvede

59 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in combination with a database extension generated in this study with 51 collection strains from 16 genera. Most strains were identified by using both databases individually, and some were identified only by applying the combined database. Thus, the methodology is very useful and the generated database extension was helpful.
Original languageEnglish
JournalJournal of Clinical Microbiology
Volume50
Issue number5
Pages (from-to)1787-91
Number of pages5
ISSN0095-1137
DOIs
Publication statusPublished - 2012

Keywords

  • Bacteriological Techniques
  • Catalase
  • Gram-Positive Cocci
  • Software
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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