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Mannan-binding lectin in diabetic kidney disease: the impact of mouse genetics in a type 1 diabetes model

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Østergaard, JA, Bjerre, M, RamachandraRao, SP, Sharma, K, Nyengaard, JR, Hansen, TK, Thiel, S & Flyvbjerg, A 2012, 'Mannan-binding lectin in diabetic kidney disease: the impact of mouse genetics in a type 1 diabetes model' Experimental Diabesity Research, vol. 2012, pp. 678381. https://doi.org/10.1155/2012/678381

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Østergaard, Jakob Appel ; Bjerre, Mette ; RamachandraRao, Satish Posettihalli ; Sharma, Kumar ; Nyengaard, Jens Randel ; Hansen, Troels Krarup ; Thiel, Steffen ; Flyvbjerg, Allan. / Mannan-binding lectin in diabetic kidney disease : the impact of mouse genetics in a type 1 diabetes model. In: Experimental Diabesity Research. 2012 ; Vol. 2012. pp. 678381.

Bibtex

@article{315d9c1545ba44ff91661f1e73e2c5f0,
title = "Mannan-binding lectin in diabetic kidney disease: the impact of mouse genetics in a type 1 diabetes model",
abstract = "UNLABELLED: BACKGROUND. Mannan-binding lectin (MBL) is involved in the development of diabetic nephropathy. MBL is a part of the innate immune system where it can activate the complement system. Serum MBL level predicts later renal impairment in diabetes patients. Direct involvement of MBL in the development of diabetic kidney disease is observed in one animal strain. However, this involvement may differ among the animal strains. We thus examined the impact of the genetic background on the role of MBL in diabetic nephropathy.MATERIALS/METHODS: C57BL/6JBomTac and 129S6/SvEvTac mice were compared. In both strains, experimental type 1 diabetes was induced in wild-type (WT) and MBL-knockout (MBL-KO) mice by streptozotocin. Nondiabetic WT and MBL-KO mice were used as controls. We tested if MBL modified the diabetes-induced kidney changes by two-way ANOVA allowing for interaction.RESULTS: MBL aggravated diabetes-induced kidney growth and glomerulus enlargement in C57BL/6JBomTac mice. MBL did not modify diabetes effects on glomerular basement membrane thickness or mesangial volume in any strain. Diabetes-induced changes in renal gene transcription of growth factors and matrix components were unaffected by MBL.CONCLUSIONS: Strain-specific MBL effects were found on downstream diabetic kidney changes. This emphasizes the importance of genetic background in this model of diabetic complications.",
keywords = "Analysis of Variance, Animals, Blood Glucose, Body Weight, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1, Diabetic Nephropathies, Disease Models, Animal, Female, Gene Expression Regulation, Kidney, Mannose-Binding Lectin, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Genetic, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't",
author = "{\O}stergaard, {Jakob Appel} and Mette Bjerre and RamachandraRao, {Satish Posettihalli} and Kumar Sharma and Nyengaard, {Jens Randel} and Hansen, {Troels Krarup} and Steffen Thiel and Allan Flyvbjerg",
year = "2012",
doi = "10.1155/2012/678381",
language = "English",
volume = "2012",
pages = "678381",
journal = "Experimental Diabesity Research",
issn = "1543-8600",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Mannan-binding lectin in diabetic kidney disease

T2 - the impact of mouse genetics in a type 1 diabetes model

AU - Østergaard, Jakob Appel

AU - Bjerre, Mette

AU - RamachandraRao, Satish Posettihalli

AU - Sharma, Kumar

AU - Nyengaard, Jens Randel

AU - Hansen, Troels Krarup

AU - Thiel, Steffen

AU - Flyvbjerg, Allan

PY - 2012

Y1 - 2012

N2 - UNLABELLED: BACKGROUND. Mannan-binding lectin (MBL) is involved in the development of diabetic nephropathy. MBL is a part of the innate immune system where it can activate the complement system. Serum MBL level predicts later renal impairment in diabetes patients. Direct involvement of MBL in the development of diabetic kidney disease is observed in one animal strain. However, this involvement may differ among the animal strains. We thus examined the impact of the genetic background on the role of MBL in diabetic nephropathy.MATERIALS/METHODS: C57BL/6JBomTac and 129S6/SvEvTac mice were compared. In both strains, experimental type 1 diabetes was induced in wild-type (WT) and MBL-knockout (MBL-KO) mice by streptozotocin. Nondiabetic WT and MBL-KO mice were used as controls. We tested if MBL modified the diabetes-induced kidney changes by two-way ANOVA allowing for interaction.RESULTS: MBL aggravated diabetes-induced kidney growth and glomerulus enlargement in C57BL/6JBomTac mice. MBL did not modify diabetes effects on glomerular basement membrane thickness or mesangial volume in any strain. Diabetes-induced changes in renal gene transcription of growth factors and matrix components were unaffected by MBL.CONCLUSIONS: Strain-specific MBL effects were found on downstream diabetic kidney changes. This emphasizes the importance of genetic background in this model of diabetic complications.

AB - UNLABELLED: BACKGROUND. Mannan-binding lectin (MBL) is involved in the development of diabetic nephropathy. MBL is a part of the innate immune system where it can activate the complement system. Serum MBL level predicts later renal impairment in diabetes patients. Direct involvement of MBL in the development of diabetic kidney disease is observed in one animal strain. However, this involvement may differ among the animal strains. We thus examined the impact of the genetic background on the role of MBL in diabetic nephropathy.MATERIALS/METHODS: C57BL/6JBomTac and 129S6/SvEvTac mice were compared. In both strains, experimental type 1 diabetes was induced in wild-type (WT) and MBL-knockout (MBL-KO) mice by streptozotocin. Nondiabetic WT and MBL-KO mice were used as controls. We tested if MBL modified the diabetes-induced kidney changes by two-way ANOVA allowing for interaction.RESULTS: MBL aggravated diabetes-induced kidney growth and glomerulus enlargement in C57BL/6JBomTac mice. MBL did not modify diabetes effects on glomerular basement membrane thickness or mesangial volume in any strain. Diabetes-induced changes in renal gene transcription of growth factors and matrix components were unaffected by MBL.CONCLUSIONS: Strain-specific MBL effects were found on downstream diabetic kidney changes. This emphasizes the importance of genetic background in this model of diabetic complications.

KW - Analysis of Variance

KW - Animals

KW - Blood Glucose

KW - Body Weight

KW - Diabetes Mellitus, Experimental

KW - Diabetes Mellitus, Type 1

KW - Diabetic Nephropathies

KW - Disease Models, Animal

KW - Female

KW - Gene Expression Regulation

KW - Kidney

KW - Mannose-Binding Lectin

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Models, Genetic

KW - Transcription, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1155/2012/678381

DO - 10.1155/2012/678381

M3 - Journal article

VL - 2012

SP - 678381

JO - Experimental Diabesity Research

JF - Experimental Diabesity Research

SN - 1543-8600

ER -

ID: 51724428