Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
E-pub ahead of print

Localization of TRPA1 channels and characterization of TRPA1 mediated responses in dural and pial arteries in vivo after intracarotid infusion of Na2S

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Cellular distribution of PACAP-38 and PACAP receptors in the rat brain: Relation to migraine activated regions

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Ictal neck pain investigated in the interictal state - a search for the origin of pain

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Persistent post-traumatic headache attributed to mild traumatic brain injury: Deep phenotyping and treatment patterns

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Letter to the editor regarding proposed new diagnostic criteria for migraine

    Research output: Contribution to journalLetterResearchpeer-review

  5. Eptinezumab in episodic migraine: A randomized, double-blind, placebo-controlled study (PROMISE-1)

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Letter to the editor regarding proposed new diagnostic criteria for migraine

    Research output: Contribution to journalLetterResearchpeer-review

  2. Sumatriptan Does Not Antagonize CGRP-Induced Symptoms in Healthy Volunteers

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Von Frey testing revisited - provision of an online algorithm for improved accuracy of 50% thresholds

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Familial analysis reveals rare risk variants for migraine in regulatory regions

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Genetic identification of cell types underlying brain complex traits yields insights into the etiology of Parkinson’s disease

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

BACKGROUND: The Transient Receptor Potential Ankyrin 1 (TRPA1) channel might play a role in migraine. However, different mechanisms for this have been suggested. The purpose of our study was to investigate the localization and significance of TRPA1 channels in rat pial and dural arteries.

METHODS: Immunofluorescence microscopy was used to localize TRPA1 channels in dural arteries, pial arteries, dura mater and trigeminal ganglion. The genuine closed cranial window model was used to examine the effect of Na2S, a donor of the TRPA1 channel opener H2S, on the diameter of pial and dural arteries. Further, we performed blocking experiments with TRPA1 antagonist HC-030031, calcitonin gene-related peptide (CGRP) receptor antagonist olcegepant and KCa3.1 channel blocker TRAM-34.

RESULTS: TRPA1 channels were localized to the endothelium of both dural and pial arteries and in nerve fibers in dura mater. Further, we found TRPA1 expression in the membrane of trigeminal ganglia neuronal cells, some of them also staining for CGRP. Na2S caused dilation of both dural and pial arteries. In dural arteries, this was inhibited by HC-030031 and olcegepant. In pial arteries, the dilation was inhibited by TRAM-34, suggesting involvement of the KCa3.1 channel.

CONCLUSION: Na2S causes a TRPA1- and CGRP-dependent dilation of dural arteries and a KCa3.1 channel-dependent dilation of pial arteries in rats.

Original languageEnglish
JournalCephalalgia : an international journal of headache
Pages (from-to)333102420937724
ISSN0333-1024
DOIs
Publication statusE-pub ahead of print - 1 Jul 2020

    Research areas

  • cerebral arteries, genuine closed cranial window model, meningeal arteries, migraine, sodium sulfide, Transient receptor potential ankyrin 1

ID: 60284467