TY - JOUR
T1 - Liquid Biopsies in Follicular Thyroid Carcinomas-A Brief Report
AU - Hansen, Marie-Louise Uhre
AU - Bendtsen, Simone Kloch
AU - Jakobsen, Kathrine Kronberg
AU - Schmidt, Ane Yde
AU - Hahn, Christoffer Holst
AU - von Buchwald, Christian
AU - Grønhøj, Christian
PY - 2024/7/22
Y1 - 2024/7/22
N2 - Thyroid cancer (TC) represents a significant health burden globally, with follicular thyroid cancer (FTC) posing diagnostic challenges despite advancements. This pilot study aimed to evaluate the utility of a liquid biopsy with cell-free DNA (cfDNA) in patients with FTC. Blood samples were collected from 13 patients diagnosed with FTC, DNA extraction was performed, and cfDNA was analyzed using the Illumina's TruSight Oncology 500 High-Throughput panel. The results revealed low tumor mutational burden and minimal pathogenic variants in cfDNA, indicating challenges such as low DNA yield and poor material quality despite adequate coverage. Our findings indicate that cfDNA as an add-on diagnostic tool in patients with FTC might not be a useful supplement.
AB - Thyroid cancer (TC) represents a significant health burden globally, with follicular thyroid cancer (FTC) posing diagnostic challenges despite advancements. This pilot study aimed to evaluate the utility of a liquid biopsy with cell-free DNA (cfDNA) in patients with FTC. Blood samples were collected from 13 patients diagnosed with FTC, DNA extraction was performed, and cfDNA was analyzed using the Illumina's TruSight Oncology 500 High-Throughput panel. The results revealed low tumor mutational burden and minimal pathogenic variants in cfDNA, indicating challenges such as low DNA yield and poor material quality despite adequate coverage. Our findings indicate that cfDNA as an add-on diagnostic tool in patients with FTC might not be a useful supplement.
UR - http://www.scopus.com/inward/record.url?scp=85199752253&partnerID=8YFLogxK
M3 - Journal article
C2 - 39061714
SN - 2075-4418
VL - 14
JO - Diagnostics
JF - Diagnostics
IS - 14
M1 - 1577
ER -