@inbook{5feaf2d8ad604d83bccf6a671776af05,
title = "Ligand Identification for Orphan MHC-Agnostic T-Cell Receptors by Whole Genome CRISPR–Cas9 Screening",
abstract = "Killer T-cells play important roles in immunity to infection and cancer by detecting intracellular anomalies at the cell surface and destroying the cells that bear them. Conventional killer T-cells scan the intracellular proteome by sampling peptides presented at the cell surface by major histocompatibility complex (MHC) molecules. It is becoming apparent that some T-cells can also respond to pathogens and neoplasms by sensing intracellular changes through molecules other than MHC. We describe an unbiased methodology for T-cell receptor ligand discovery that requires no a priori knowledge regarding the nature of the antigen.",
keywords = "CRISPR, Lentivirus, Ligand discovery, MHC-independent, MR1, T-cell, Major Histocompatibility Complex, Receptors, Antigen, T-Cell/genetics, CRISPR-Cas Systems, Histocompatibility Antigens, Ligands",
author = "Crowther, {Michael D.} and Mateusz Legut and Sewell, {Andrew K.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2712-9_1",
language = "English",
volume = "2574",
series = "Methods in Molecular Biology",
publisher = "Humana Press, Inc",
pages = "3--14",
booktitle = "Methods in Molecular Biology",
}