Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Isotope-dilution TurboFlow-LC-MS/MS method for simultaneous quantification of ten steroid metabolites in serum

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Analysis of HOXA9 methylated ctDNA in ovarian cancer using sense-antisense measurement

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Citrullination of fibrinogen by peptidylarginine deiminase 2 impairs fibrin clot structure

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Evaluation of algorithm development approaches: Development of biomarker panels for early detection of colorectal lesions

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Bilateral oophorectomy and rate of colorectal cancer: A prospective cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Vitamin D supplementation improves fasting insulin levels and HDL cholesterol in infertile men

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Treatment options for hypercalcemia after cosmetic oil injections: Lessons from human tissue cultures and a pilot intervention study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Endocrine Disrupting Chemicals and Risk of Testicular Cancer: A Systematic Review and Meta-analysis

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Calcium transport in male reproduction is possibly influenced by vitamin D and CaSR

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

An isotope-dilution TurboFlow-LC-MS/MS method for simultaneous quantification of the ten steroid metabolites dehydroepiandrosterone sulfate (DHEAS), progesterone, 17α-hydroxyprogesterone (17-OHP), Δ4-androstenedione (Adione), corticosterone, 11-deoxycortisol, cortisol, cortisone, testosterone (T), and estrone 3-sulfate (E1-S) in serum was developed and validated. Limits of quantification, variability (inter- and intra-day), analytical range and linearity were all found to be acceptable for clinical use. Furthermore, sample stability was evaluated including the influence of freeze-thaw cycles and the effects of temperature and storage time. The method was applied to 391 serum samples from healthy, Danish boys 10-18years old. The concentration ranges of the included steroid metabolites for this population are presented. Concentrations of DHEAS, 17-OHP, Adione and T in the 391 serum samples were furthermore compared to results obtained using an existing LC-MS/MS method in our laboratory. Excellent agreement was found between the methods. Furthermore, the improved sensitivity of the new method allowed for quantification of a number of samples found to be below the LOQs of the existing method. Thus, the two instruments and their associated methods were validated as possible back-ups for each other, which we consider an extremely important issue in high-throughput laboratories analyzing clinical samples on a regular basis. The ten analytes included can be analyzed simultaneously but it is also possible only to include some of these analytes for specific diagnostic purposes which make the new method an extremely useful tool in the clinical laboratory.

Original languageEnglish
JournalClinica chimica acta; international journal of clinical chemistry
Volume468
Pages (from-to)180-186
Number of pages7
ISSN0009-8981
DOIs
Publication statusPublished - 2017

    Research areas

  • Journal Article

ID: 50123540