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Investigation of tissue inhibitor of metalloproteinases 1 in plasma from colorectal cancer patients and blood donors by surface-enhanced laser desorption/ionization time-of-flight mass spectrometryscopy.

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  1. Crystal Structures of Human C4.4A Reveal the Unique Association of Ly6/uPAR/α-neurotoxin Domain

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  2. Tissue inhibitor of metalloproteinases-1 as a biological marker 
in colorectal cancer: influence of smoking on plasma levels?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Aberrantly methylated DNA as a biomarker in breast cancer

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  4. Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1

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  5. Levels of tissue inhibitor of metalloproteinases 1 in plasma and urine from patients with cancer

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  1. Triage May Improve Selection to Colonoscopy and Reduce the Number of Unnecessary Colonoscopies

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  2. Early Detection and Recurrence of Colorectal Adenomas by Combination of Eight Cancer-Associated Biomarkers in Plasma

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  3. Gel-Based Proteomics of Clinical Samples Identifies Potential Serological Biomarkers for Early Detection of Colorectal Cancer

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Early detection of colorectal cancer (CRC) improves patient survival. Plasma tissue inhibitor of metalloproteinases 1 (TIMP-1) measurements by enzyme-linked immunosorbent assay (ELISA) have been suggested as a new method for the early detection of CRC. To further investigate the nature of TIMP-1 in plasma, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF MS) was used. TIMP-1 measurements of plasma from 16 healthy donors and 14 CRC patients were performed using TIMP-1 monoclonal antibody in SELDI TOF MS and ELISA. SELDI TOF MS applying an antibody to TIMP-1 revealed that human plasma TIMP-1 has a mass of 25.1 kDa and exhibits several isoforms. Both methods showed increased plasma TIMP-1 values for cancer patients as compared to healthy individuals. The p values for the separation of the groups were 0.0019 for ELISA and <0.0001 for SELDI TOF MS. CRC did not fundamentally affect the appearance of TIMP-1 as evaluated by SELDI TOF MS.
Original languageEnglish
JournalInternational Journal of Biological Markers
Volume22
Issue number2
Pages (from-to)89-94
Number of pages6
ISSN0393-6155
Publication statusPublished - 2007

ID: 32498898