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Investigating circadian clock gene expression in human tendon biopsies from acute exercise and immobilization studies

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@article{53e4b67494184ce09cdbc64d3d29bed3,
title = "Investigating circadian clock gene expression in human tendon biopsies from acute exercise and immobilization studies",
abstract = "PURPOSE: The discovery of musculoskeletal tissues, including muscle, tendons, and cartilage, as peripheral circadian clocks strongly implicates their role in tissue-specific homeostasis. Age-related dampening and misalignment of the tendon circadian rhythm and its outputs may be responsible for the decline in tendon homeostasis. It is unknown which entrainment signals are responsible for the synchronization of the tendon clock to the light-dark cycle.METHODS: We sought to examine any changes in the expression levels of core clock genes (BMAL1, CLOCK, PER2, CRY1, and NR1D1) in healthy human patellar tendon biopsies obtained from three different intervention studies: increased physical activity (leg kicks for 1 h) in young, reduced activity (2 weeks immobilization of one leg) in young, and in old tendons.RESULTS: The expression level of clock genes in human tendon in vivo was very low and a high variation between individuals was found. We were thus unable to detect any differences in core clock gene expression neither after acute exercise nor immobilization.CONCLUSIONS: We are unable to find evidence for an effect of exercise or immobilization on circadian clock gene expression in human tendon samples.",
keywords = "Adult, Aged, Circadian Rhythm Signaling Peptides and Proteins/genetics, Exercise, Humans, Immobilization/adverse effects, Male, Patellar Ligament/growth & development",
author = "Yeung, {Ching-Yan Chlo{\'e}} and Peter Schjerling and Heinemeier, {Katja M} and Boesen, {Anders P} and Kasper Dideriksen and Michael Kj{\ae}r",
year = "2019",
month = jun,
doi = "10.1007/s00421-019-04129-2",
language = "English",
volume = "119",
pages = "1387--1394",
journal = "European Journal of Applied Physiology and Occupational Physiology",
issn = "1439-6319",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Investigating circadian clock gene expression in human tendon biopsies from acute exercise and immobilization studies

AU - Yeung, Ching-Yan Chloé

AU - Schjerling, Peter

AU - Heinemeier, Katja M

AU - Boesen, Anders P

AU - Dideriksen, Kasper

AU - Kjær, Michael

PY - 2019/6

Y1 - 2019/6

N2 - PURPOSE: The discovery of musculoskeletal tissues, including muscle, tendons, and cartilage, as peripheral circadian clocks strongly implicates their role in tissue-specific homeostasis. Age-related dampening and misalignment of the tendon circadian rhythm and its outputs may be responsible for the decline in tendon homeostasis. It is unknown which entrainment signals are responsible for the synchronization of the tendon clock to the light-dark cycle.METHODS: We sought to examine any changes in the expression levels of core clock genes (BMAL1, CLOCK, PER2, CRY1, and NR1D1) in healthy human patellar tendon biopsies obtained from three different intervention studies: increased physical activity (leg kicks for 1 h) in young, reduced activity (2 weeks immobilization of one leg) in young, and in old tendons.RESULTS: The expression level of clock genes in human tendon in vivo was very low and a high variation between individuals was found. We were thus unable to detect any differences in core clock gene expression neither after acute exercise nor immobilization.CONCLUSIONS: We are unable to find evidence for an effect of exercise or immobilization on circadian clock gene expression in human tendon samples.

AB - PURPOSE: The discovery of musculoskeletal tissues, including muscle, tendons, and cartilage, as peripheral circadian clocks strongly implicates their role in tissue-specific homeostasis. Age-related dampening and misalignment of the tendon circadian rhythm and its outputs may be responsible for the decline in tendon homeostasis. It is unknown which entrainment signals are responsible for the synchronization of the tendon clock to the light-dark cycle.METHODS: We sought to examine any changes in the expression levels of core clock genes (BMAL1, CLOCK, PER2, CRY1, and NR1D1) in healthy human patellar tendon biopsies obtained from three different intervention studies: increased physical activity (leg kicks for 1 h) in young, reduced activity (2 weeks immobilization of one leg) in young, and in old tendons.RESULTS: The expression level of clock genes in human tendon in vivo was very low and a high variation between individuals was found. We were thus unable to detect any differences in core clock gene expression neither after acute exercise nor immobilization.CONCLUSIONS: We are unable to find evidence for an effect of exercise or immobilization on circadian clock gene expression in human tendon samples.

KW - Adult

KW - Aged

KW - Circadian Rhythm Signaling Peptides and Proteins/genetics

KW - Exercise

KW - Humans

KW - Immobilization/adverse effects

KW - Male

KW - Patellar Ligament/growth & development

U2 - 10.1007/s00421-019-04129-2

DO - 10.1007/s00421-019-04129-2

M3 - Journal article

C2 - 30923873

VL - 119

SP - 1387

EP - 1394

JO - European Journal of Applied Physiology and Occupational Physiology

JF - European Journal of Applied Physiology and Occupational Physiology

SN - 1439-6319

IS - 6

ER -

ID: 59363134