Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Local recurrence rate in a national Danish patient cohort after curative treatment for rectal cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Circulating allergen-specific T2 lymphocytes: CCR4rather than CLAis the predominant phenotype in peanut-allergic subjects

    Research output: Contribution to journalComment/debateResearchpeer-review

  3. EAACI Guidelines on allergen immunotherapy: IgE-mediated food allergy

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Clinical relevance of sensitization to hydrolyzed wheat protein in wheat-sensitized subjects

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Food allergy: setting the scene for tolerance induction

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Rie Satoh
  • Reiko Teshima
  • Kazumi Kitta
  • Gang-Hua Lang
  • Kathleen Schegg
  • Kenneth Blumenthal
  • Leslie Hicks
  • Bénédicte Labory-Carcenac
  • David Rouquié
  • Rod A Herman
  • Corinne Herouet-Guicheney
  • Gregory S Ladics
  • Scott McClain
  • Lars K Poulsen
  • Laura Privalle
  • Jason M Ward
  • Nancy Doerrer
  • Jean-Baptiste Rascle
View graph of relations

In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14-16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.

Original languageEnglish
JournalBioscience, biotechnology, and biochemistry
Volume80
Issue number11
Pages (from-to)2198-2207
Number of pages10
ISSN0916-8451
DOIs
Publication statusPublished - 11 Jul 2016

    Research areas

  • Journal Article

ID: 49902014