Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Insulin signaling in skeletal muscle of HIV-infected patients in response to endurance and strength training

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Increased oral sodium chloride intake in humans amplifies selectively postprandial GLP-1 but not GIP, CCK, and gastrin in plasma

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Changes in metabolism but not myocellular signaling by training with CHO-restriction in endurance athletes

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. High-intensity interval training changes mitochondrial respiratory capacity differently in adipose tissue and skeletal muscle

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Silent Hypoxia in Patients with SARS CoV-2 Infection before Hospital Discharge

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Svær adenoviruspneumoni hos en immunkompetent mand

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
Human immunodeficiency virus (HIV)-infected patients with lipodystrophy have decreased insulin-stimulated glucose uptake. Both endurance and resistance training improve insulin-stimulated glucose uptake in skeletal muscle of HIV-infected patients, but the mechanisms are unknown. This study aims to identify the molecular pathways involved in the beneficial effects of training on insulin-stimulated glucose uptake in skeletal muscle of HIV-infected patients. Eighteen sedentary male HIV-infected patients underwent a 16 week supervised training intervention, either resistance or strength training. Euglycemic-hyperinsulinemic clamps with muscle biopsies were performed before and after the training interventions. Fifteen age- and body mass index (BMI)-matched HIV-negative men served as a sedentary baseline group. Phosphorylation and total protein expression of insulin signaling molecules as well as glycogen synthase (GS) activity were analyzed in skeletal muscle biopsies in relation to insulin stimulation before and after training. HIV-infected patients had reduced basal and insulin-stimulated GS activity (%fractional velocity, [FV]) as well as impaired insulin-stimulated Akt(thr308) phosphorylation. Despite improving insulin-stimulated glucose uptake, neither endurance nor strength training changed the phosphorylation status of insulin signaling proteins or affected GS activity. However; endurance training markedly increased the total Akt protein expression, and both training modalities increased hexokinase II (HKII) protein. HIV-infected patients with lipodystrophy have decreased insulin-stimulated glucose uptake in skeletal muscle and defects in insulin-stimulated phosphorylation of Akt(thr308). Endurance and strength training increase insulin-stimulated glucose uptake in these patients, and the muscular training adaptation is associated with improved capacity for phosphorylation of glucose by HKII, rather than changes in markers of insulin signaling to glucose uptake or glycogen synthesis.
Original languageEnglish
JournalPhysiological Reports
Volume1
Issue number3
Pages (from-to)e00060
DOIs
Publication statusPublished - Aug 2013

ID: 43144223