In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark

Rasmus K Hare; Jan B Gertsen; Karen M T Astvad; Kristine B Degn; Anders Løkke; Marc Stegger; Paal S Andersen; Lise Kristensen; Maiken C Arendrup

doi:10.3201/eid2503.180297

In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark

Rasmus K Hare, Jan B Gertsen, Karen M T Astvad, Kristine B Degn, Anders Løkke, Marc Stegger, Paal S Andersen, Lise Kristensen, Maiken C Arendrup

49 Citations (Scopus)

Abstract

We report a fatal aspergillosis case in which STRAf typing and whole-genome sequencing substantiated in vivo emergence of an azole-resistant Aspergillus fumigatus with a 120-bp tandem repeat in the promoter region of cyp51A. This event, previously restricted to the environment, challenges current understanding of azole resistance development in A. fumigatus.

Original language	English
Journal	Emerging Infectious Diseases (Print Edition)
Volume	25
Issue number	3
Pages (from-to)	577-580
Number of pages	4
ISSN	1080-6040
DOIs	https://doi.org/10.3201/eid2503.180297
Publication status	Published - Mar 2019

Keywords

Aspergillosis/diagnosis
Aspergillus fumigatus/drug effects
Azoles/pharmacology
Cytochrome P-450 Enzyme System/genetics
Denmark/epidemiology
Drug Resistance, Fungal
Fungal Proteins/genetics
Humans
Male
Middle Aged
Mutation
Phylogeny
Promoter Regions, Genetic
Selection, Genetic
Tandem Repeat Sequences
Tomography, X-Ray Computed

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10.3201/eid2503.180297

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Hare, R. K., Gertsen, J. B., Astvad, K. M. T., Degn, K. B., Løkke, A., Stegger, M., Andersen, P. S., Kristensen, L., & Arendrup, M. C. (2019). In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark. Emerging Infectious Diseases (Print Edition), 25(3), 577-580. https://doi.org/10.3201/eid2503.180297

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abstract = "We report a fatal aspergillosis case in which STRAf typing and whole-genome sequencing substantiated in vivo emergence of an azole-resistant Aspergillus fumigatus with a 120-bp tandem repeat in the promoter region of cyp51A. This event, previously restricted to the environment, challenges current understanding of azole resistance development in A. fumigatus.",

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doi = "10.3201/eid2503.180297",

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pages = "577--580",

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AU - Gertsen, Jan B

AU - Astvad, Karen M T

AU - Degn, Kristine B

AU - Løkke, Anders

AU - Stegger, Marc

AU - Andersen, Paal S

AU - Kristensen, Lise

AU - Arendrup, Maiken C

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AB - We report a fatal aspergillosis case in which STRAf typing and whole-genome sequencing substantiated in vivo emergence of an azole-resistant Aspergillus fumigatus with a 120-bp tandem repeat in the promoter region of cyp51A. This event, previously restricted to the environment, challenges current understanding of azole resistance development in A. fumigatus.

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KW - Aspergillus fumigatus/drug effects

KW - Azoles/pharmacology

KW - Cytochrome P-450 Enzyme System/genetics

KW - Denmark/epidemiology

KW - Drug Resistance, Fungal

KW - Fungal Proteins/genetics

KW - Humans

KW - Male

KW - Middle Aged

KW - Mutation

KW - Phylogeny

KW - Promoter Regions, Genetic

KW - Selection, Genetic

KW - Tandem Repeat Sequences

KW - Tomography, X-Ray Computed

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SN - 1080-6040

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EP - 580

JO - Emerging Infectious Diseases (Print Edition)

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In Vivo Selection of a Unique Tandem Repeat Mediated Azole Resistance Mechanism (TR120) in Aspergillus fumigatus cyp51A, Denmark

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