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In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

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@article{d4cd70ad544f4482ab7d159bd3a82908,
title = "In Vitro Neutralization Assay Using Cultured Hepatitis C Virus",
abstract = "The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.",
keywords = "Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine",
author = "Jannick Prentoe and Jens Bukh",
year = "2019",
doi = "10.1007/978-1-4939-8976-8_29",
language = "English",
volume = "1911",
pages = "433--439",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press, Inc",

}

RIS

TY - JOUR

T1 - In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

AU - Prentoe, Jannick

AU - Bukh, Jens

PY - 2019

Y1 - 2019

N2 - The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

AB - The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

KW - Antibodies

KW - HCV

KW - Hepatitis C virus

KW - Immunostaining

KW - Neutralization

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=85059235929&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8976-8_29

DO - 10.1007/978-1-4939-8976-8_29

M3 - Journal article

C2 - 30593643

VL - 1911

SP - 433

EP - 439

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 56060778