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In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

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  1. Equine pegiviruses cause persistent infection of bone marrow and are not associated with hepatitis

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  2. Evolutionary selection of pestivirus variants with altered or no microRNA dependency

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  3. Antibody Responses to Immunization With HCV Envelope Glycoproteins as a Baseline for B cell-Based Vaccine Development

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The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

Original languageEnglish
JournalMethods in molecular biology
Volume1911
Pages (from-to)433-439
Number of pages7
ISSN1064-3745
DOIs
Publication statusPublished - 2019

    Research areas

  • Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine

ID: 56060778