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The Capital Region of Denmark - a part of Copenhagen University Hospital
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In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

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  1. HCV p7 as a novel vaccine-target inducing multifunctional CD4+ and CD8+ T-cells targeting liver cells expressing the viral antigen

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  2. Replicons of a rodent hepatitis C model virus permit selection of highly permissive cells

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  3. Evolutionary Pathways to Persistence of Highly Fit and Resistant Hepatitis C Virus Protease Inhibitor Escape Variants

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  4. Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations

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The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

Original languageEnglish
JournalMethods in molecular biology (Clifton, N.J.)
Volume1911
Pages (from-to)433-439
Number of pages7
ISSN1064-3745
DOIs
Publication statusPublished - 2019

    Research areas

  • Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine

ID: 56060778