Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Analysis of Mass Cytometry Data

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Noninvasive Antenatal Screening for Fetal RHD in RhD Negative Women to Guide Targeted Anti-D Prophylaxis

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

Original languageEnglish
JournalMethods in molecular biology
Volume1911
Pages (from-to)433-439
Number of pages7
ISSN1064-3745
DOIs
Publication statusPublished - 2019

    Research areas

  • Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine

ID: 56060778