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The Capital Region of Denmark - a part of Copenhagen University Hospital
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In Vitro Maturation and Culture of Human Oocytes

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  4. Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries

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We describe the collection, culture, and ex vivo, in vitro maturation of germinal vesicle (GV) oocytes obtained from human small antral follicles (hSAFs). hSAFs contain fully grown GV oocytes and have the advantages that they are more numerous than large or mature follicles, which are used in IVF treatment. hSAFs can be obtained directly from human ovarian tissue without exogenous gonadotrophin stimulation and therefore allows studies of oocytes even from young women and girls. The method described here was developed to study human female meiosis but could in theory also be used for fertility treatment.

Original languageEnglish
JournalMethods in molecular biology
Volume1818
Pages (from-to)23-30
Number of pages8
ISSN1064-3745
DOIs
Publication statusPublished - 2018

ID: 54944633