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Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

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Hultqvist, Louise Dahl ; Alhede, Maria ; Jakobsen, Tim Holm ; Givskov, Michael ; Bjarnsholt, Thomas. / Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo. In: Methods in molecular biology (Clifton, N.J.). 2018 ; Vol. 1673. pp. 203-212.

Bibtex

@article{51157289561f45ed9b44ef166b438c60,
title = "Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo",
abstract = "In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.",
keywords = "Journal Article",
author = "Hultqvist, {Louise Dahl} and Maria Alhede and Jakobsen, {Tim Holm} and Michael Givskov and Thomas Bjarnsholt",
year = "2018",
doi = "10.1007/978-1-4939-7309-5_16",
language = "English",
volume = "1673",
pages = "203--212",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press, Inc",

}

RIS

TY - JOUR

T1 - Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

AU - Hultqvist, Louise Dahl

AU - Alhede, Maria

AU - Jakobsen, Tim Holm

AU - Givskov, Michael

AU - Bjarnsholt, Thomas

PY - 2018

Y1 - 2018

N2 - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

AB - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

KW - Journal Article

U2 - 10.1007/978-1-4939-7309-5_16

DO - 10.1007/978-1-4939-7309-5_16

M3 - Journal article

VL - 1673

SP - 203

EP - 212

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 52090106