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Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

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In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

Original languageEnglish
JournalMethods in molecular biology (Clifton, N.J.)
Volume1673
Pages (from-to)203-212
Number of pages10
ISSN1064-3745
DOIs
Publication statusPublished - 2018

    Research areas

  • Journal Article

ID: 52090106