Identification of thioredoxin target disulfides in proteins released from barley aleurone layers

Per Hägglund, Jakob Bunkenborg, Fen Yang, Lea Mørch Harder, Christine Finnie, Birte Svensson

21 Citations (Scopus)

Abstract

Thioredoxins are ubiquitous disulfide reductases involved in a wide range of cellular processes including DNA synthesis, oxidative stress response and apoptosis. In cereal seeds thioredoxins are proposed to facilitate the germination process by reducing disulfide bonds in storage proteins and other targets in the starchy endosperm. Here we have applied a thiol-specific labeling approach to identify specific disulfide targets of barley thioredoxin in proteins released from barley aleurone layers incubated in buffer containing gibberellic acid.
Original languageEnglish
JournalJournal of Proteomics
Volume73
Issue number6
Pages (from-to)1133-6
Number of pages4
ISSN1874-3919
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Buffers
  • DNA
  • Disulfides
  • Endosperm
  • Gene Expression Regulation, Plant
  • Germination
  • Gibberellins
  • Hordeum
  • Plant Leaves
  • Plant Proteins
  • Proteomics
  • Seeds
  • Thioredoxins

Fingerprint

Dive into the research topics of 'Identification of thioredoxin target disulfides in proteins released from barley aleurone layers'. Together they form a unique fingerprint.

Cite this