Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

The effect of storage conditions on salivary cortisol concentrations using an enzyme immunoassay

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Platelet function in lung cancer patients undergoing lobectomy

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Platelet increment is not associated with endothelial damage in haematological patients: a prospective observational study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Change in HbA1c concentration as decision parameter for frequency of HbA1c measurement

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Relation between invasive hemodynamics and measured glomerular filtration rate by 51Cr-EDTA clearance in advanced heart failure

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Validation of plasma thyroxine and triiodothyronine methods on the ADVIA Centaur® XP

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Optimization of preprocessing strategies in Positron Emission Tomography (PET) neuroimaging: A [11C]DASB PET study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Association Between Sumatriptan Treatment During a Migraine Attack and Central 5-HT1B Receptor Binding

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Genome-wide gene expression in a pharmacological hormonal transition model and its relation to depressive symptoms

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Three weeks of SSRI administration enhances the visual perceptual threshold - a randomized placebo-controlled study

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Correction: Psychedelic effects of psilocybin correlate with serotonin 2A receptor occupancy and plasma psilocin levels

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Saliva samples are easy to collect and are applicable for home-sampling, e.g. when studying HPA-axis dynamics to characterize diurnal cortisol profiles and the cortisol awakening response. However, the storing and transport conditions might be critical in the home-sampling approach. Here, we tested the stability of saliva cortisol in samples stored at different temperatures and after repeated thawing-freezing cycles when measured with an Enzyme Immuno Assay (EIA). Thirteen healthy volunteers, six women and seven men, mean age 31 (range 26-49) years collected saliva either in the morning hours (08:00-10:00 h) or before lunch (11:00-12:00 h). Storage at six different conditions were tested: Storage at - 18°C, - 4°C, 4°C and room temperature for 72 h. One condition tested was at - 18°C for 72 h and then kept in an envelope for 72 h with a freezing element in room temperature surroundings where after it was stored at - 80°C. The last tube was stored directly at - 80°C and served as the 'gold standard'. The saliva samples were assayed using Salivary Cortisol Diagnostic EIA. Differences in cortisol measurements between each of the five conditions and the 'gold standard' (- 80°C) were evaluated by one-sample t-test. No significant differences were observed. This indicates that an EIA method can be used reliably when measuring salivary cortisol samples obtained by home-sampling including a postal delivery.

Original languageEnglish
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume75
Issue number1
Pages (from-to)92-5
Number of pages4
ISSN0036-5513
DOIs
Publication statusPublished - Jan 2015

ID: 45589024