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The Capital Region of Denmark - a part of Copenhagen University Hospital
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The effect of storage conditions on salivary cortisol concentrations using an enzyme immunoassay

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Saliva samples are easy to collect and are applicable for home-sampling, e.g. when studying HPA-axis dynamics to characterize diurnal cortisol profiles and the cortisol awakening response. However, the storing and transport conditions might be critical in the home-sampling approach. Here, we tested the stability of saliva cortisol in samples stored at different temperatures and after repeated thawing-freezing cycles when measured with an Enzyme Immuno Assay (EIA). Thirteen healthy volunteers, six women and seven men, mean age 31 (range 26-49) years collected saliva either in the morning hours (08:00-10:00 h) or before lunch (11:00-12:00 h). Storage at six different conditions were tested: Storage at - 18°C, - 4°C, 4°C and room temperature for 72 h. One condition tested was at - 18°C for 72 h and then kept in an envelope for 72 h with a freezing element in room temperature surroundings where after it was stored at - 80°C. The last tube was stored directly at - 80°C and served as the 'gold standard'. The saliva samples were assayed using Salivary Cortisol Diagnostic EIA. Differences in cortisol measurements between each of the five conditions and the 'gold standard' (- 80°C) were evaluated by one-sample t-test. No significant differences were observed. This indicates that an EIA method can be used reliably when measuring salivary cortisol samples obtained by home-sampling including a postal delivery.

Original languageEnglish
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume75
Issue number1
Pages (from-to)92-5
Number of pages4
ISSN0036-5513
DOIs
Publication statusPublished - Jan 2015

ID: 45589024