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Immune Monitoring Using mRNA-Transfected Dendritic Cells

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Dendritic cells are known to be the most potent antigen presenting cell in the immune system and are used as cellular adjuvants in therapeutic anticancer vaccines using various tumor-associated antigens or their derivatives. One way of loading antigen into the dendritic cells is by mRNA electroporation, ensuring presentation of antigen through major histocompatibility complex I and potentially activating T cells, enabling them to kill the tumor cells. Despite extensive research in the field, only one dendritic cell-based vaccine has been approved. There is therefore a great need to elucidate and understand the immunological impact of dendritic cell vaccination in order to improve clinical benefit. In this chapter, we describe a method for performing immune monitoring using peripheral blood mononuclear cells and autologous dendritic cells transfected with tumor-associated antigen-encoding mRNA.

Original languageEnglish
JournalMethods in molecular biology (Clifton, N.J.)
Volume1428
Pages (from-to)245-59
Number of pages15
ISSN1064-3745
DOIs
Publication statusPublished - 2016

    Research areas

  • Journal Article

ID: 49640145