Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Multiple genes encode the major surface glycoprotein of Pneumocystis carinii

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Site-specific O-glycosylation of members of the low-density lipoprotein receptor superfamily enhances ligand interactions

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Profound Endothelial Damage Predicts Impending Organ Failure and Death in Sepsis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The Danish Microbiology Database (MiBa) 2010 to 2013

    Research output: Contribution to journalJournal articleResearchpeer-review

  • J A Kovacs
  • F Powell
  • J C Edman
  • B Lundgren
  • Angel Ricardo Martinez
  • B Drew
  • C W Angus
View graph of relations
The major surface antigen of Pneumocystis carinii, a life-threatening opportunistic pathogen in human immunodeficiency virus-infected patients, is an abundant glycoprotein that functions in host-organism interactions. A monoclonal antibody to this antigen is protective in animals, and thus this antigen is a good candidate for development as a vaccine to prevent or control P. carinii infection. We have cloned and sequenced seven related but unique genes encoding the major surface glycoprotein of rat P. carinii. Partial amino acid sequencing confirmed the identity of these genes. Based on Southern blot studies using chromosomal or restricted DNA, the major surface glycoproteins are the products of a multicopy family of genes. The predicted protein has an M(r) of approximately 123,000, is relatively rich in cysteine residues (5.5%) that are very strongly conserved, and contains a well conserved hydrophobic region at the carboxyl terminus. The presence of multiple related msg genes encoding the major surface glycoprotein of P. carinii suggests that antigenic variation is a possible mechanism for evading host defenses. Further characterization of this family of genes should allow the development of novel approaches to the control of this pathogen.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume268
Issue number8
Pages (from-to)6034-40
Number of pages7
ISSN0021-9258
Publication statusPublished - 15 Mar 1993

    Research areas

  • Amino Acid Sequence, Animals, Antigens, Surface, Base Sequence, DNA, Fungal, Genes, Fungal, Membrane Glycoproteins, Molecular Sequence Data, Multigene Family, Pneumocystis, Rats, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid

ID: 39874590