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Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

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  1. The effects of season and weather on healthcare utilization among patients with atopic dermatitis

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  2. Changes in filaggrin degradation products and corneocyte surface texture by season

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  3. The association between atopic dermatitis and hand eczema: a systematic review and meta-analysis

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  4. Effect of atopic skin stressors on natural moisturizing factors and cytokines in healthy adult epidermis

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  • S A Koppes
  • P Kemperman
  • I van Tilburg
  • F Calkoen-Kwa
  • K A Engebretsen
  • G J Puppels
  • P J Caspers
  • S Kezic
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BACKGROUND: Natural moisturizing factors (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC.

RESULTS: Measured NMF values were significantly correlated (R2 = 0.61; P < 0.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicate robustness and good reproducibility of both methods.

CONCLUSION: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.

Original languageEnglish
JournalBiomarkers
Volume22
Issue number6
Pages (from-to)502-507
ISSN1354-750X
DOIs
Publication statusPublished - 18 Aug 2017

ID: 49271791