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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Diagnosis of pulmonary infection with Toxoplasma gondii in immunocompromised HIV-positive patients by real-time PCR

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The aim of the study presented here was to evaluate the use of PCR for improving the diagnosis of Toxoplasma gondii infection in immunocompromised hosts. Three hundred thirty-two bronchoalveolar lavage (BAL) fluid samples were analyzed by real-time PCR targeting a 529 bp element of T. gondii. In positive samples, the genotype of the parasite was determined by sequence analysis of the GRA6 gene. Positive results were achieved for 2% (7/332) of the samples tested. Genotyping was possible in two samples and revealed GRA6 type II T. gondii. PCR for detecting T. gondii in BAL samples should be performed in all immunosuppressed HIV-positive patients with symptoms of a systemic infection of unknown etiology. Trimethoprim-sulfamethoxazole prophylaxis does not exclude concomitant infection with T. gondii.
Original languageEnglish
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Volume25
Issue number6
Pages (from-to)401-4
Number of pages3
ISSN0934-9723
DOIs
Publication statusPublished - 2006

    Research areas

  • AIDS-Related Opportunistic Infections, Animals, Bronchoalveolar Lavage Fluid, Genotype, Humans, Lung Diseases, Parasitic, Parasitology, Polymerase Chain Reaction, Toxoplasma, Toxoplasmosis

ID: 32503230