Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Quantitative autoradiography of ligands for dopamine receptors and transporters in brain of Göttingen minipig: comparison with results in vivo

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Similar serotonin-2A receptor binding in rats with different coping styles or levels of aggression

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Neuropeptide Y-stimulated [(35) S]GTPγs functional binding is reduced in the hippocampus after kainate-induced seizures in mice

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Acute social defeat does not alter cerebral 5-HT2A receptor binding in male Wistar rats

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Transient activation of mTOR following forced treadmill exercise in rats

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Transdiagnostic group CBT for anxiety disorders: the unified protocol in mental health services

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Cognitive behavioural therapy for ADHD in adults: systematic review and meta-analyses

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Et læringsteoretisk perspektiv på klinisk mesterlære

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Postdischarge interventions for depression (Protocol)

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Self-disorder and Brain Processing of Proprioception in Schizophrenia Spectrum Patients: A Re-analysis

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Luciano Minuzzi
  • Aage Kristian Olsen Alstrup
  • Dirk Bender
  • Sidse Arnfred
  • Rebecca Grant
  • Erik Hvid Danielsen
  • Paul Cumming
View graph of relations
The pig has been used as animal model for positron emission tomography (PET) studies of dopamine (DA) receptors and pharmacological perturbations of DA neurotransmission. However, the binding properties of DA receptors and transporters in pig brain have not been characterized in vitro. Therefore, the saturation binding parameters of [3H]SCH 23390 for DA D1 receptors and [3H]raclopride for DA D2/3 receptors were measured by quantitative autoradiography in cryostat sections from brain of groups of 8 week old and adult female Göttingen minipigs. The magnitudes of Bmax and Kd for these ligands were similar in young and old pigs, and were close to those reported for rat and human brain. Furthermore, gradients in the concentrations of D1 and D2/3 sites in striatum measured in vitro agreed with earlier findings in PET studies. However, the dopamine transporter (DAT) ligand [3H]GBR12935 did not bind in pig brain cryostat sections. Whereas the tropane derivative [125I]RTI-55 labeled serotonin transporters (serotonin transporter (SERT)) in pig brain, use of the same ligand under conditions specific for DAT, revealed a pattern of binding similar to that observed for SERT conditions. Parallel studies revealed the presence of DAT in rat and ferret brain. The distribution volume (Vd) of the selective DAT ligand [11C]NS2214 ([11C]Brasofensine) was mapped in groups of normal and MPTP-lesioned Göttingen miniature pigs. The in vivo pattern of Vd matched the distribution of SERT in vitro, and did not differ between the normal pigs and the lesioned animals with documented 60% DA depletions. However, the pattern of specific binding of the selective noradrenaline transporter ligand (S,S)-[11C]MeNER in a single Landrace pig showed that, of the three monoamine transporters, only DAT could not be detected in pig brain. We conclude that the pig is a suitable model for PET studies of DA D1 and D2/3 binding sites, which are fully developed on the eighth postnatal week. However, well-characterized piperazine and tropane radioligands failed to recognize DAT in pig brain; the two tropane radioligands lacked pharmacological specificity for DAT and SERT in pig brain in vitro and in vivo.
Original languageEnglish
JournalSynapse (New York)
Volume59
Issue number4
Pages (from-to)211-9
Number of pages9
ISSN0887-4476
DOIs
StatePublished - 15 Mar 2006

    Research areas

  • Age Factors, Animals, Autoradiography, Binding, Competitive, Brain, Cocaine, Dopamine Antagonists, Dopamine Plasma Membrane Transport Proteins, Female, Ferrets, Humans, Ligands, Piperazines, Positron-Emission Tomography, Raclopride, Radiopharmaceuticals, Rats, Receptors, Dopamine, Swine, Swine, Miniature

ID: 32753000