Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

A novel method for long-term monitoring of intracranial pressure in rats

Research output: Research - peer-reviewJournal article

DOI

  1. Automatic delineation of brain regions on MRI and PET images from the pig

    Research output: Research - peer-reviewJournal article

  2. Nerve excitability in the rat forelimb: a technique to improve translational utility

    Research output: Research - peer-reviewJournal article

View graph of relations

BACKGROUND: In preclinical neurological studies, monitoring intracranial pressure (ICP) in animal models especially in rodents is challenging. Further, the lack of methods for long-term ICP monitoring has limited the possibilities to conduct prolonged studies on ICP fluctuations in parallel to disease progression or therapeutic interventions. For these reasons we aimed to set up a simple and valid method for long-term ICP recordings in rats.

NEW METHOD: A novel ICP method employing epidural probes was developed and validated by simultaneously ICP recordings in the lateral ventricle and in the epidural space. The two pressures were recorded twice a week for 59 days and the correlation was studied.

RESULTS: The two pressure recordings correlated exceptionally well and the R(2) values on each recording day ranged between 0.99 and 1.00. However, the ventricular probes caused a number of complications including loss of patency and tissue damage probably due to cerebral infection, whereas the epidural probes were safe and reliable throughout the entire study.

COMPARISON WITH EXISTING METHODS: Epidural probes are much easier to implant than ventricular probes. In addition, these new probes are far less invasive and induce no apparent mechanical tissue damage and highly decrease the infection risk associated with ICP recordings.

CONCLUSION: Epidural ICP recorded with this new method is identical to the ventricular ICP for at least 59 days but is far less complicated and safer for the animals. The long-term method described is reliable, valid, inexpensive, and may be used in multiple disease models to study ICP.

Original languageEnglish
JournalJournal of Neuroscience Methods
Volume227
Pages (from-to)1-9
Number of pages9
ISSN0165-0270
DOIs
StatePublished - 30 Apr 2014

    Research areas

  • Animals, Blood Pressure, Epidural Space, Female, Intracranial Pressure, Lateral Ventricles, Linear Models, Male, Monitoring, Physiologic, Rats, Rats, Sprague-Dawley, Reproducibility of Results, Time Factors

ID: 44891653