Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

No association between body mass index and sperm DNA integrity

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Patients' attitudes and preferences towards a freeze-all strategy in ART treatment

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Recurrent pregnancy loss: couples' perspectives on their need for treatment, support and follow up

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Anogenital distance is associated with semen quality but not reproductive hormones in 1106 young men from the general population

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Cumulative live birth rate prognosis based on the number of aspirated oocytes in previous ART cycles

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Decrease in semen quality and Leydig cell function in infertile men: a longitudinal study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Forebyggelse og behandling af cytomegalovirusinfektion hos gravide og nyfødte

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Night work and miscarriage: a Danish nationwide register-based cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Wet work exposure: comparison of observed and self-reported data

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Night work and sick leave during pregnancy: a national register-based within-worker cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  • I Bandel
  • M Bungum
  • J Richtoff
  • J Malm
  • J Axelsson
  • H S Pedersen
  • J K Ludwicki
  • K Czaja
  • A Hernik
  • G Toft
  • J P Bonde
  • M Spanò
  • G Malm
  • T B Haugen
  • A Giwercman
View graph of relations

STUDY QUESTION: Is overweight associated with impaired sperm DNA integrity?

SUMMARY ANSWER: High body mass index (BMI) is not associated with impaired sperm DNA integrity as assessed by the DNA Fragmentation Index (DFI).

WHAT IS KNOWN ALREADY: Previous studies, based on fewer subjects and including mainly subfertile men, have shown conflicting results regarding the influence of overweight and obesity on sperm DNA integrity.

STUDY DESIGN, SIZE, DURATION: This cross-sectional study was based on semen samples from 1503 men from the general population.

PARTICIPANTS/MATERIALS, SETTING, METHODS: We included two cohorts (cohort A and B) of military recruits (n = 275, n = 304, respectively), one group (cohort C) of fertile men and men without known fertility problems (n = 724), and one group (cohort D) of men between 19 and 40 years without known fertility problems (n = 200). In all cohorts, data were available on BMI, DFI as measured by the sperm chromatin structure assay (SCSA), standard semen characteristics, and potential confounders (age, abstinence time, smoking habits). The subjects were categorized according to BMI into four groups: underweight (<18.5 kg/m(2)), normal weight (18.5-24.9 kg/m(2)), overweight (25.0-29.9 kg/m(2)) and obese (≥30.0 kg/m(2)). Using a linear regression model, the inter-group differences in DFI were calculated. Furthermore with the normal-weight group as the reference, the odds ratios (ORs) for DFI > 20% and DFI > 30%, were calculated for the other groups. Calculations were made for the material as a whole and after exclusion of cohort C which included proven fertile men.

MAIN RESULTS AND THE ROLE OF CHANCE: We found that normal-weight men had significantly higher DFI than overweight men, with a mean difference of 1.13% (95% CI: 1.05-1.22%); P = 0.001). Overweight men had a reduced risk of having DFI ≥ 20% and DFI ≥ 30%, compared with normal-weight men; adjusted odds ratio (OR) = 0.61 (95% CI: 0.42-0.88; P < 0.01) and adjusted OR = 0.48 (95% CI: 0.28-0.84; P < 0.01), respectively. When excluding cohort C, the statistical significance was lost. Regarding standard semen parameters, we found that obese men had a higher percentage of progressive motile spermatozoa than normal-weight men; mean difference 1.15% (95% CI: 1.02-1.30%, P < 0.05) but the significance was lost when excluding cohort C. All other standard semen parameters were unaffected by BMI.

LIMITATIONS, REASONS FOR CAUTION: A main limitation might be the cross-sectional nature of the data. Furthermore our study included a significant proportion of men with proven fertility (75% of cohort C, n = 550), and could therefore be biased toward fertility.

WIDER IMPLICATIONS OF THE FINDINGS: Our study indicates that overweight per se is not associated with a higher level of sperm DNA damage.

STUDY FUNDING/COMPETING INTERESTS: This research has been given grants from the following: EU 5th and 7th framework program (Inuendo and Clear projects, [Contracts no. QLK4-CT-2001-00202 and FP7-ENV-2008-1-226217)]), the Swedish Research Council (Grants No. 2007-2590, 521-2004-6072 and 521-2002-3907); the Swedish Governmental Funding for Clinical Research, Skåne county council's research and development foundation, MAS Funds, University Hospital MAS Foundation in Malmö, Crafoordska Fund, Ove Tulefjords Fund, Foundation for Urological Research, Fundacion Federico SA, and Gunnar Nilssons Cancer Fund. The authors declare that there are no conflicts of interest.

Original languageEnglish
JournalHuman reproduction (Oxford, England)
Volume30
Issue number7
Pages (from-to)1704-13
Number of pages10
ISSN0268-1161
DOIs
Publication statusPublished - Jul 2015

ID: 45882019