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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Contraction and AICAR stimulate IL-6 vesicle depletion from skeletal muscle fibers in vivo

Publication: Research - peer-reviewJournal article

DOI

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Recent studies suggest that interleukin 6 (IL-6) is released from contracting skeletal muscles; however, the cellular origin, secretion kinetics, and signaling mechanisms regulating IL-6 secretion are unknown. To address these questions, we developed imaging methodology to study IL-6 in fixed mouse muscle fibers and in live animals in vivo. Using confocal imaging to visualize endogenous IL-6 protein in fixed muscle fibers, we found IL-6 in small vesicle structures distributed throughout the fibers under basal (resting) conditions. To determine the kinetics of IL-6 secretion, intact quadriceps muscles were transfected with enhanced green fluorescent protein (EGFP)-tagged IL-6 (IL-6-EGFP), and 5 days later anesthetized mice were imaged before and after muscle contractions in situ. Contractions decreased IL-6-EGFP-containing vesicles and protein by 62% (P
Original languageEnglish
JournalDiabetes
Volume62
Issue number9
Pages (from-to)3081-92
Number of pages12
ISSN0012-1797
DOIs
StatePublished - Sep 2013

    Research areas

  • Aminoimidazole Carboxamide, Animals, Green Fluorescent Proteins, Interleukin-6, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Confocal, Muscle Contraction, Muscle Fibers, Skeletal, Ribonucleotides

ID: 42221656