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Mutations in ZMYND10, a gene essential for proper axonemal assembly of inner and outer dynein arms in humans and flies, cause primary ciliary dyskinesia

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    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Quantifying the Impact of Rare and Ultra-rare Coding Variation across the Phenotypic Spectrum

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  3. Life-Course Genome-wide Association Study Meta-analysis of Total Body BMD and Assessment of Age-Specific Effects

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  • Daniel J Moore
  • Alexandros Onoufriadis
  • Amelia Shoemark
  • Michael A Simpson
  • Petra I zur Lage
  • Sandra C de Castro
  • Lucia Bartoloni
  • Giuseppe Gallone
  • Stavroula Petridi
  • Wesley J Woollard
  • Dinu Antony
  • Miriam Schmidts
  • Teresa Didonna
  • Periklis Makrythanasis
  • Jeremy Bevillard
  • Nigel P Mongan
  • Jana Djakow
  • Gerard Pals
  • Jane S Lucas
  • June K Marthin
  • Kim G Nielsen
  • Federico Santoni
  • Michel Guipponi
  • Claire Hogg
  • Stylianos E Antonarakis
  • Richard D Emes
  • Eddie M K Chung
  • Nicholas D E Greene
  • Jean-Louis Blouin
  • Andrew P Jarman
  • Hannah M Mitchison
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Primary ciliary dyskinesia (PCD) is a ciliopathy characterized by airway disease, infertility, and laterality defects, often caused by dual loss of the inner dynein arms (IDAs) and outer dynein arms (ODAs), which power cilia and flagella beating. Using whole-exome and candidate-gene Sanger resequencing in PCD-affected families afflicted with combined IDA and ODA defects, we found that 6/38 (16%) carried biallelic mutations in the conserved zinc-finger gene BLU (ZMYND10). ZMYND10 mutations conferred dynein-arm loss seen at the ultrastructural and immunofluorescence level and complete cilia immotility, except in hypomorphic p.Val16Gly (c.47T>G) homozygote individuals, whose cilia retained a stiff and slowed beat. In mice, Zmynd10 mRNA is restricted to regions containing motile cilia. In a Drosophila model of PCD, Zmynd10 is exclusively expressed in cells with motile cilia: chordotonal sensory neurons and sperm. In these cells, P-element-mediated gene silencing caused IDA and ODA defects, proprioception deficits, and sterility due to immotile sperm. Drosophila Zmynd10 with an equivalent c.47T>G (p.Val16Gly) missense change rescued mutant male sterility less than the wild-type did. Tagged Drosophila ZMYND10 is localized primarily to the cytoplasm, and human ZMYND10 interacts with LRRC6, another cytoplasmically localized protein altered in PCD. Using a fly model of PCD, we conclude that ZMYND10 is a cytoplasmic protein required for IDA and ODA assembly and that its variants cause ciliary dysmotility and PCD with laterality defects.

Original languageEnglish
JournalAmerican Journal of Human Genetics
Volume93
Issue number2
Pages (from-to)346-56
Number of pages11
DOIs
StatePublished - 8 Aug 2013

    Research areas

  • Animals, Axoneme, Cilia, Drosophila melanogaster, Dyneins, Exome, Female, Gene Expression Regulation, High-Throughput Nucleotide Sequencing, Humans, Infertility, Male, Kartagener Syndrome, Male, Mice, Mutation, Pedigree, Protein Structure, Tertiary, Proteins, Respiratory System, Tumor Suppressor Proteins

ID: 43625539