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The Capital Region of Denmark - a part of Copenhagen University Hospital
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The effect of non-esterified long-chain fatty acids on blood flow and thermogenesis in brown adipose tissue in the young dog

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  1. Analysis of ET-A and ET-B receptors using an isolated perfused rat lung preparation

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  2. Intra- and peri-tendinous microdialysis determination of glucose and lactate in pigs

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  3. In situ microdialysis of intramuscular prostaglandin and thromboxane in contracting skeletal muscle in humans

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In vitro experiments have demonstrated that increasing the molar ratio of extracellular non-esterified fatty acids (NEFA) to albumin stimulates thermogenesis in brown adipocytes. To test these results, in vivo blood flow and local temperature were measured in perirenal brown adipose tissue (BAT) in puppies during thermogenesis induced by increasing the plasma NEFA: albumin ratio by injection of intralipid and heparin. Plasma NEFA concentration increased to 1.5 mmol X 1-I and plasma NEFA:albumin ratio to 4. Concomitantly, the whole body oxygen consumption rose on average about 100% above baseline level. Plasma noradrenaline concentration increased about three-fold and plasma adrenaline concentration about six-fold. The BAT temperature increased by an average of 0.9 degrees C. However, since BAT blood flow was simultaneously reduced by about 50%, it can be calculated that the local heat production was also reduced. Consequently, the increase in whole body oxygen consumption was not due to stimulation of BAT thermogenesis. It is concluded that in vivo assessment of BAT thermogenesis requires concomitant measurements of both local BAT temperature and blood flow.
Original languageEnglish
JournalActa Physiologica Scandinavica. Supplementum
Volume124
Issue number1
Pages (from-to)81-5
Number of pages5
ISSN0302-2994
DOIs
Publication statusPublished - May 1985

    Research areas

  • Adipose Tissue, Adipose Tissue, Brown, Animals, Blood Glucose, Blood Pressure, Body Temperature Regulation, Catecholamines, Dogs, Fat Emulsions, Intravenous, Fatty Acids, Nonesterified, Heparin, Oxygen Consumption, Pulse, Regional Blood Flow, Serum Albumin

ID: 39027812