Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Lipoprotein(a): the common, likely causal, yet elusive risk factor for cardiovascular disease

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Weekday variation in triglyceride concentrations in 1.8 million blood samples

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Mobility of HSPG-bound LPL explains how LPL is able to reach GPIHBP1 on capillaries

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Monoclonal Antibodies That Bind to the Ly6 Domain of GPIHBP1 Abolish the Binding of LPL

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Soluble urokinase plasminogen activator receptor predicts cardiovascular events, mortality and kidney function decline in patients with type 1 diabetes

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  2. Cellular homeostatic tension and force transmission measured in human engineered tendon

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Carbon-14 bomb pulse dating shows that tendinopathy is preceded by years of abnormally high collagen turnover

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Effect of Losartan on the Acute Response of Human Elderly Skeletal Muscle to Exercise

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
The aim of this study was to investigate the molecular mechanisms regulating FA translocase CD36 (FAT/CD36) translocation and FA uptake in skeletal muscle during contractions. In one model, wild-type (WT) and AMP-dependent protein kinase kinase dead (AMPK KD) mice were exercised or extensor digitorum longus (EDL) and soleus (SOL) muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and FA uptake in response to muscle contractions were investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, 5-aminoimidazole-4-carboxamide ribonucleoside only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions was associated with increased FA uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and FA uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.
Original languageEnglish
JournalJournal of Lipid Research
Volume52
Issue number4
Pages (from-to)699-711
Number of pages13
ISSN0022-2275
DOIs
Publication statusPublished - 2011

    Research areas

  • AMP-Activated Protein Kinases, Aminoimidazole Carboxamide, Animals, Antigens, CD36, Biological Transport, Fatty Acids, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Muscle Contraction, Muscle, Skeletal, Physical Conditioning, Animal, Protein Transport, Rats, Ribonucleosides

ID: 34730983