Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Activation of the interleukin-6/Janus kinase/STAT3 pathway in pleomorphic adenoma of the parotid gland

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Primary antibiotic resistance of Helicobacter pylori strains among adults and children in a tertiary referral centre in Lithuania

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. A short history of microbial biofilms and biofilm infections

    Research output: Contribution to journalReviewResearchpeer-review

  3. Biofilms and host response - helpful or harmful

    Research output: Contribution to journalReviewResearchpeer-review

  4. Diagnosis of biofilm infections in cystic fibrosis patients

    Research output: Contribution to journalReviewResearchpeer-review

  1. PET/CT prior to salvage surgery in recurrent head and neck squamous cell carcinoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Intratumor heterogeneity of PD-L1 expression in head and neck squamous cell carcinoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Salivary gland carcinomas with unusual presentations

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Adherence to preventive swallowing exercises for head and neck cancer patients undergoing (chemo)radiotherapy treatment

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Answer to: "Is the National Danish Colorectal Cancer Screening Programme a success?"

    Research output: Contribution to journalJournal articleResearch

View graph of relations

The interleukin-6 (IL-6)/Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) pathway is of crucial importance in promoting tumorigenesis in several malignant tumors but may also be active in benign tumors, e.g., of pleomorphic adenoma (PA). In this study we characterize the expression of the pathway components with immunohistochemistry and selected mRNAs and microRNAs (miRNAs) regulated by this pathway in isolated duct- and myoepithelial cells in PA. 46 PAs were immunostained and 10 of these were used for in situ hybridization (ISH). Six frozen specimens were analyzed using reverse transcription-polymerase chain reaction (RT-PCR). Using immunohistochemistry, IL-6, JAK1, JAK2 and STAT3 were detected significantly more frequently in PA cells than in cells from normal salivary gland tissue. Using RT-PCR cyclin D1, fibroblast growth factor 2, and p21 were found to be overexpressed while matrix metallopeptidase 9 was detected at low levels in PA compared to normal salivary gland. ISH showed significant overexpression of miR-181b in PA, while miR-21 was undetectable in PA and normal tissue. Overexpression of the pathway components and its mRNA and miRNA products provide important clues regarding the growth of PAs. Our findings brings us one step closer to targeted treatment of this tumor entity, although in vitro studies are warranted to confirm this.

Original languageEnglish
JournalAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Volume123
Issue number8
Pages (from-to)706-15
Number of pages10
ISSN0903-4641
DOIs
Publication statusPublished - Aug 2015

    Research areas

  • Adenoma, Pleomorphic, Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Immunohistochemistry, Interleukin-6, Janus Kinase 1, Janus Kinase 2, Male, MicroRNAs, Middle Aged, Parotid Gland, RNA, Messenger, STAT3 Transcription Factor, Signal Transduction, Young Adult

ID: 46000784