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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Hypoxia-regulated MicroRNAs in Gastroesophageal Cancer

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BACKGROUND/AIM: The present study aimed to identify hypoxia-regulated microRNAs (HRMs) in vitro and investigate the clinical role of candidate HRMs in patients with gastroesophageal cancer (GEC).

MATERIALS AND METHODS: microRNA expression changes induced by hypoxia in human GEC cell lines were measured with microarrays and validated by quantitative real-time polymerase chain reaction. Candidate HRMs were measured in pre-therapeutic tumor samples from 195 patients with GEC.

RESULTS: Expression of miR-210 was shown to be significantly induced in esophageal squamous cell carcinoma (9.26-fold, p<0.001) and adenocarcinoma cell lines (4.95-fold, p<0.001) and miR-27a-star was significantly up-regulated in adenocarcinoma cell lines (4.79-fold, p=0.04). A weak but significant correlation between miR-210 expression and a 15-gene hypoxia signature was observed (Pearson r correlation: r=0.38, p<0.001). No significant associations of HRMs and clinical outcome in patients with GEC were identified.

CONCLUSION: This study supports the involvement of hypoxia on miRNAs in vitro and confirms the role of miR-210 as being a universal HRM.

Original languageEnglish
JournalAnticancer Research
Volume36
Issue number2
Pages (from-to)721-30
Number of pages10
ISSN0250-7005
Publication statusPublished - Feb 2016

    Research areas

  • Adenocarcinoma, Adult, Aged, Aged, 80 and over, Carcinoma, Squamous Cell, Cell Hypoxia, Cell Line, Tumor, Esophageal Neoplasms, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Kaplan-Meier Estimate, Male, MicroRNAs, Middle Aged, Oligonucleotide Array Sequence Analysis, Proportional Hazards Models, Real-Time Polymerase Chain Reaction, Retrospective Studies, Treatment Outcome, Journal Article, Research Support, Non-U.S. Gov't

ID: 49686262