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Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels

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Harvard

Bang, L, Nielsen-Kudsk, JE, Gruhn, N, Trautner, S, Theilgaard, SA, Olesen, SP, Boesgaard, S & Aldershvile, J 1998, 'Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels' European Journal of Pharmacology, vol. 361, no. 1, pp. 43-9.

APA

Bang, L., Nielsen-Kudsk, J. E., Gruhn, N., Trautner, S., Theilgaard, S. A., Olesen, S. P., ... Aldershvile, J. (1998). Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels. European Journal of Pharmacology, 361(1), 43-9.

CBE

MLA

Vancouver

Author

Bang, Lone ; Nielsen-Kudsk, J E ; Gruhn, N ; Trautner, S ; Theilgaard, S A ; Olesen, S P ; Boesgaard, S ; Aldershvile, J. / Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels. In: European Journal of Pharmacology. 1998 ; Vol. 361, No. 1. pp. 43-9.

Bibtex

@article{d992913b3d944f2e9aa19d2cc7855259,
title = "Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels",
abstract = "The purpose of this study was to investigate whether high conductance Ca2+-activated K+ channels (BK(Ca)) are mediating the vasodilator action of hydralazine. In isolated porcine coronary arteries, hydralazine (1-300 microM), like the K+ channel opener levcromakalim, preferentially relaxed contractions induced by K+ (20 mM) compared with K+ (80 mM). In addition, concentration-relaxation curves for hydralazine (pD2 = 5.38 +/- 0.06; Emax = 85.9 +/- 3.6{\%}) were shifted 10-fold to the right by the BK(Ca) blockers tetraethylammonium (1 mM) and iberiotoxin (0.1 microM). In contrast, nimodipine (a Ca2+-entry blocker), relaxed contractions induced by K+ (20 mM) and K+ (80 mM) equally and nimodipine-induced relaxations were neither antagonized by tetraethylammonium nor by iberiotoxin. In isolated perfused rat hearts, hydralazine (1 microM) increased coronary flow by 28.8 +/- 2.7{\%}. Iberiotoxin (0.1 microM) suppressed this response by 82{\%} (P < 0.05). In conscious, chronically catheterized rats the hypotensive response to hydralazine (0.6 mg kg(-1) min(-1)) was significantly reduced by 41{\%} during infusion of iberiotoxin (0.1 mg kg(-1)). It is concluded, that opening of BK(Ca) takes part in the mechanism whereby hydralazine produces vasodilation.",
keywords = "Animals, Blood Pressure, Calcium, Carcinogens, Cattle, Consciousness, Coronary Vessels, Cromakalim, Dose-Response Relationship, Drug, Electrophysiology, Heart, Hydrazines, In Vitro Techniques, Male, Nimodipine, Patch-Clamp Techniques, Peptides, Potassium, Potassium Channels, Rats, Swine, Tetraethylammonium, Vasodilation, Vasodilator Agents",
author = "Lone Bang and Nielsen-Kudsk, {J E} and N Gruhn and S Trautner and Theilgaard, {S A} and Olesen, {S P} and S Boesgaard and J Aldershvile",
year = "1998",
month = "11",
day = "13",
language = "English",
volume = "361",
pages = "43--9",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier BV",
number = "1",

}

RIS

TY - JOUR

T1 - Hydralazine-induced vasodilation involves opening of high conductance Ca2+-activated K+ channels

AU - Bang, Lone

AU - Nielsen-Kudsk, J E

AU - Gruhn, N

AU - Trautner, S

AU - Theilgaard, S A

AU - Olesen, S P

AU - Boesgaard, S

AU - Aldershvile, J

PY - 1998/11/13

Y1 - 1998/11/13

N2 - The purpose of this study was to investigate whether high conductance Ca2+-activated K+ channels (BK(Ca)) are mediating the vasodilator action of hydralazine. In isolated porcine coronary arteries, hydralazine (1-300 microM), like the K+ channel opener levcromakalim, preferentially relaxed contractions induced by K+ (20 mM) compared with K+ (80 mM). In addition, concentration-relaxation curves for hydralazine (pD2 = 5.38 +/- 0.06; Emax = 85.9 +/- 3.6%) were shifted 10-fold to the right by the BK(Ca) blockers tetraethylammonium (1 mM) and iberiotoxin (0.1 microM). In contrast, nimodipine (a Ca2+-entry blocker), relaxed contractions induced by K+ (20 mM) and K+ (80 mM) equally and nimodipine-induced relaxations were neither antagonized by tetraethylammonium nor by iberiotoxin. In isolated perfused rat hearts, hydralazine (1 microM) increased coronary flow by 28.8 +/- 2.7%. Iberiotoxin (0.1 microM) suppressed this response by 82% (P < 0.05). In conscious, chronically catheterized rats the hypotensive response to hydralazine (0.6 mg kg(-1) min(-1)) was significantly reduced by 41% during infusion of iberiotoxin (0.1 mg kg(-1)). It is concluded, that opening of BK(Ca) takes part in the mechanism whereby hydralazine produces vasodilation.

AB - The purpose of this study was to investigate whether high conductance Ca2+-activated K+ channels (BK(Ca)) are mediating the vasodilator action of hydralazine. In isolated porcine coronary arteries, hydralazine (1-300 microM), like the K+ channel opener levcromakalim, preferentially relaxed contractions induced by K+ (20 mM) compared with K+ (80 mM). In addition, concentration-relaxation curves for hydralazine (pD2 = 5.38 +/- 0.06; Emax = 85.9 +/- 3.6%) were shifted 10-fold to the right by the BK(Ca) blockers tetraethylammonium (1 mM) and iberiotoxin (0.1 microM). In contrast, nimodipine (a Ca2+-entry blocker), relaxed contractions induced by K+ (20 mM) and K+ (80 mM) equally and nimodipine-induced relaxations were neither antagonized by tetraethylammonium nor by iberiotoxin. In isolated perfused rat hearts, hydralazine (1 microM) increased coronary flow by 28.8 +/- 2.7%. Iberiotoxin (0.1 microM) suppressed this response by 82% (P < 0.05). In conscious, chronically catheterized rats the hypotensive response to hydralazine (0.6 mg kg(-1) min(-1)) was significantly reduced by 41% during infusion of iberiotoxin (0.1 mg kg(-1)). It is concluded, that opening of BK(Ca) takes part in the mechanism whereby hydralazine produces vasodilation.

KW - Animals

KW - Blood Pressure

KW - Calcium

KW - Carcinogens

KW - Cattle

KW - Consciousness

KW - Coronary Vessels

KW - Cromakalim

KW - Dose-Response Relationship, Drug

KW - Electrophysiology

KW - Heart

KW - Hydrazines

KW - In Vitro Techniques

KW - Male

KW - Nimodipine

KW - Patch-Clamp Techniques

KW - Peptides

KW - Potassium

KW - Potassium Channels

KW - Rats

KW - Swine

KW - Tetraethylammonium

KW - Vasodilation

KW - Vasodilator Agents

M3 - Journal article

VL - 361

SP - 43

EP - 49

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -

ID: 44934019