Human B cells produce chemokine CXCL10 in the presence of Mycobacterium tuberculosis specific T cells

Soren T Hoff; Ahmed M Salman; Morten Ruhwald; Pernille Ravn; Inger Brock; Nabila Elsheikh; Peter Andersen; Else Marie Agger

doi:10.1016/j.tube.2014.10.005

Human B cells produce chemokine CXCL10 in the presence of Mycobacterium tuberculosis specific T cells

Soren T Hoff, Ahmed M Salman, Morten Ruhwald, Pernille Ravn, Inger Brock, Nabila Elsheikh, Peter Andersen, Else Marie Agger

14 Citations (Scopus)

Abstract

BACKGROUND: The role of B cells in human host response to Mycobacterium tuberculosis (Mtb) infection is still controversial, but recent evidence suggest that B cell follicle like structures within the lung may influence host responses through regulation of the local cytokine environment. A candidate for such regulation could be the chemokine CXCL10. CXCL10 is mainly produced by human monocytes, but a few reports have also found CXCL10 production by human B cells. The objective of this study was to investigate CXCL10 production by human B cells in response to in vitro stimulation with Mtb antigens.

METHODOLOGY/PRINCIPAL FINDINGS: We analyzed human blood samples from 30 volunteer donors using multiparameter flow cytometry, and identified a subgroup of B cells producing CXCL10 in response to in vitro stimulation with antigens. T cells did not produce CXCL10, but CXCL10 production by B cells appeared to be mediated via IFN-γ and dependent on contact with antigen-specific T cells recognizing the antigen.

CONCLUSION: Human B cells are able to produce CXCL10 in an IFN-γ and T cell contact-dependent manner. The present findings suggest a possible mechanism through which B cells in part may influence granuloma formation in human tuberculosis (TB) and participate in infection control.

Original language	English
Journal	Tuberculosis (Edinburgh, Scotland)
Volume	95
Issue number	1
Pages (from-to)	40-7
Number of pages	8
ISSN	1472-9792
DOIs	https://doi.org/10.1016/j.tube.2014.10.005
Publication status	Published - Jan 2015

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@article{aba6a683e10743a09636c2eb8b8b7f1f,

title = "Human B cells produce chemokine CXCL10 in the presence of Mycobacterium tuberculosis specific T cells",

abstract = "BACKGROUND: The role of B cells in human host response to Mycobacterium tuberculosis (Mtb) infection is still controversial, but recent evidence suggest that B cell follicle like structures within the lung may influence host responses through regulation of the local cytokine environment. A candidate for such regulation could be the chemokine CXCL10. CXCL10 is mainly produced by human monocytes, but a few reports have also found CXCL10 production by human B cells. The objective of this study was to investigate CXCL10 production by human B cells in response to in vitro stimulation with Mtb antigens.METHODOLOGY/PRINCIPAL FINDINGS: We analyzed human blood samples from 30 volunteer donors using multiparameter flow cytometry, and identified a subgroup of B cells producing CXCL10 in response to in vitro stimulation with antigens. T cells did not produce CXCL10, but CXCL10 production by B cells appeared to be mediated via IFN-γ and dependent on contact with antigen-specific T cells recognizing the antigen.CONCLUSION: Human B cells are able to produce CXCL10 in an IFN-γ and T cell contact-dependent manner. The present findings suggest a possible mechanism through which B cells in part may influence granuloma formation in human tuberculosis (TB) and participate in infection control.",

author = "Hoff, {Soren T} and Salman, {Ahmed M} and Morten Ruhwald and Pernille Ravn and Inger Brock and Nabila Elsheikh and Peter Andersen and Agger, {Else Marie}",

year = "2015",

month = jan,

doi = "10.1016/j.tube.2014.10.005",

language = "English",

volume = "95",

pages = "40--7",

journal = "Tuberculosis (Edinburgh, Scotland)",

issn = "1472-9792",

publisher = "Churchill Livingstone",

number = "1",

}

TY - JOUR

T1 - Human B cells produce chemokine CXCL10 in the presence of Mycobacterium tuberculosis specific T cells

AU - Hoff, Soren T

AU - Salman, Ahmed M

AU - Ruhwald, Morten

AU - Ravn, Pernille

AU - Brock, Inger

AU - Elsheikh, Nabila

AU - Andersen, Peter

AU - Agger, Else Marie

PY - 2015/1

Y1 - 2015/1

N2 - BACKGROUND: The role of B cells in human host response to Mycobacterium tuberculosis (Mtb) infection is still controversial, but recent evidence suggest that B cell follicle like structures within the lung may influence host responses through regulation of the local cytokine environment. A candidate for such regulation could be the chemokine CXCL10. CXCL10 is mainly produced by human monocytes, but a few reports have also found CXCL10 production by human B cells. The objective of this study was to investigate CXCL10 production by human B cells in response to in vitro stimulation with Mtb antigens.METHODOLOGY/PRINCIPAL FINDINGS: We analyzed human blood samples from 30 volunteer donors using multiparameter flow cytometry, and identified a subgroup of B cells producing CXCL10 in response to in vitro stimulation with antigens. T cells did not produce CXCL10, but CXCL10 production by B cells appeared to be mediated via IFN-γ and dependent on contact with antigen-specific T cells recognizing the antigen.CONCLUSION: Human B cells are able to produce CXCL10 in an IFN-γ and T cell contact-dependent manner. The present findings suggest a possible mechanism through which B cells in part may influence granuloma formation in human tuberculosis (TB) and participate in infection control.

AB - BACKGROUND: The role of B cells in human host response to Mycobacterium tuberculosis (Mtb) infection is still controversial, but recent evidence suggest that B cell follicle like structures within the lung may influence host responses through regulation of the local cytokine environment. A candidate for such regulation could be the chemokine CXCL10. CXCL10 is mainly produced by human monocytes, but a few reports have also found CXCL10 production by human B cells. The objective of this study was to investigate CXCL10 production by human B cells in response to in vitro stimulation with Mtb antigens.METHODOLOGY/PRINCIPAL FINDINGS: We analyzed human blood samples from 30 volunteer donors using multiparameter flow cytometry, and identified a subgroup of B cells producing CXCL10 in response to in vitro stimulation with antigens. T cells did not produce CXCL10, but CXCL10 production by B cells appeared to be mediated via IFN-γ and dependent on contact with antigen-specific T cells recognizing the antigen.CONCLUSION: Human B cells are able to produce CXCL10 in an IFN-γ and T cell contact-dependent manner. The present findings suggest a possible mechanism through which B cells in part may influence granuloma formation in human tuberculosis (TB) and participate in infection control.

U2 - 10.1016/j.tube.2014.10.005

DO - 10.1016/j.tube.2014.10.005

M3 - Journal article

C2 - 25476870

SN - 1472-9792

VL - 95

SP - 40

EP - 47

JO - Tuberculosis (Edinburgh, Scotland)

JF - Tuberculosis (Edinburgh, Scotland)

IS - 1

ER -

Human B cells produce chemokine CXCL10 in the presence of Mycobacterium tuberculosis specific T cells

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