Human anti-rhesus D IgG1 antibody produced in transgenic plants

Thomas Bouquin; Mads Jonas Birkbak Thomsen; Leif Kofoed Nielsen; Trine Hefsgaard Green; John Mundy; Morten Hanefeld Dziegiel

Human anti-rhesus D IgG1 antibody produced in transgenic plants

Thomas Bouquin, Mads Jonas Birkbak Thomsen, Leif Kofoed Nielsen, Trine Hefsgaard Green, John Mundy, Morten Hanefeld Dziegiel

Department of Clinical Immunology

34 Citations (Scopus)

Abstract

Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

Original language	English
Journal	Transgenic Research
Volume	11
Issue number	2
Pages (from-to)	115-22
Number of pages	8
ISSN	0962-8819
Publication status	Published - Apr 2002

Keywords

Agglutination Tests
Arabidopsis
Blotting, Western
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Genetic Vectors
Humans
Immunoglobulin G
Monocytes
Plants, Genetically Modified
Recombinant Proteins
Respiratory Burst
Rh-Hr Blood-Group System

Cite this

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title = "Human anti-rhesus D IgG1 antibody produced in transgenic plants",

abstract = "Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.",

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author = "Thomas Bouquin and Thomsen, \{Mads Jonas Birkbak\} and Nielsen, \{Leif Kofoed\} and Green, \{Trine Hefsgaard\} and John Mundy and Dziegiel, \{Morten Hanefeld\}",

year = "2002",

month = apr,

language = "English",

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journal = "Transgenic Research",

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TY - JOUR

T1 - Human anti-rhesus D IgG1 antibody produced in transgenic plants

AU - Bouquin, Thomas

AU - Thomsen, Mads Jonas Birkbak

AU - Nielsen, Leif Kofoed

AU - Green, Trine Hefsgaard

AU - Mundy, John

AU - Dziegiel, Morten Hanefeld

PY - 2002/4

Y1 - 2002/4

N2 - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

AB - Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.

KW - Agglutination Tests

KW - Arabidopsis

KW - Blotting, Western

KW - Cloning, Molecular

KW - Enzyme-Linked Immunosorbent Assay

KW - Genetic Vectors

KW - Humans

KW - Immunoglobulin G

KW - Monocytes

KW - Plants, Genetically Modified

KW - Recombinant Proteins

KW - Respiratory Burst

KW - Rh-Hr Blood-Group System

UR - https://www.scopus.com/pages/publications/0036000252

M3 - Journal article

C2 - 12054345

SN - 0962-8819

VL - 11

SP - 115

EP - 122

JO - Transgenic Research

JF - Transgenic Research

IS - 2

ER -

Human anti-rhesus D IgG1 antibody produced in transgenic plants

Abstract

Keywords

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