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Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex

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@article{ec7d39a1ac23477c88322b68adbe96c4,
title = "Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex",
abstract = "The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.",
keywords = "actin, female infertility, follicle activation, Hippo pathway, ovarian cortex",
author = "Lunding, {Stine A} and Andersen, {Anders N} and Lilja Hardardottir and Olesen, {Hanna {\O}} and Kristensen, {Stine G} and Andersen, {Claus Y} and Pors, {Susanne E}",
note = "{\textcopyright} 2020 Wiley Periodicals LLC.",
year = "2020",
month = jun,
doi = "10.1002/mrd.23353",
language = "English",
volume = "87",
pages = "711--719",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "John/Wiley & Sons, Inc. John/Wiley & Sons Ltd",
number = "6",

}

RIS

TY - JOUR

T1 - Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex

AU - Lunding, Stine A

AU - Andersen, Anders N

AU - Hardardottir, Lilja

AU - Olesen, Hanna Ø

AU - Kristensen, Stine G

AU - Andersen, Claus Y

AU - Pors, Susanne E

N1 - © 2020 Wiley Periodicals LLC.

PY - 2020/6

Y1 - 2020/6

N2 - The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.

AB - The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES-associated protein upregulated nor was gene expression of the downstream growth factors CCN2, CCN3, or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.

KW - actin

KW - female infertility

KW - follicle activation

KW - Hippo pathway

KW - ovarian cortex

UR - http://www.scopus.com/inward/record.url?scp=85086044603&partnerID=8YFLogxK

U2 - 10.1002/mrd.23353

DO - 10.1002/mrd.23353

M3 - Journal article

C2 - 32506789

VL - 87

SP - 711

EP - 719

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 6

ER -

ID: 61253744