Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

Tine Rosenberg; Charlotte Aaberg-Jessen; Stine Asferg Petterson; Bjarne Winther Kristensen

doi:10.2217/cns-2017-0034

Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

Tine Rosenberg, Charlotte Aaberg-Jessen, Stine Asferg Petterson, Bjarne Winther Kristensen

7 Citations (Scopus)

Abstract

AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.

MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.

RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.

CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

Original language	English
Journal	CNS oncology
Volume	7
Issue number	2
Pages (from-to)	CNS15
ISSN	2045-0907
DOIs	https://doi.org/10.2217/cns-2017-0034
Publication status	Published - Apr 2018
Externally published	Yes

Keywords

AC133 Antigen/metabolism
Antigens, Neoplasm/metabolism
Biomarkers, Tumor/metabolism
Brain Neoplasms/metabolism
Carbonic Anhydrase IX/metabolism
Cell Hypoxia/physiology
Cell Proliferation/physiology
Gene Expression Regulation, Neoplastic
Glioblastoma/metabolism
Humans
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
Ki-67 Antigen/metabolism
Neoplastic Stem Cells/metabolism
Nestin/metabolism
Spheroids, Cellular/metabolism
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A/metabolism

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10.2217/cns-2017-0034

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title = "Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia",

abstract = "AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS \& METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2\% or 21\% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.",

keywords = "AC133 Antigen/metabolism, Antigens, Neoplasm/metabolism, Biomarkers, Tumor/metabolism, Brain Neoplasms/metabolism, Carbonic Anhydrase IX/metabolism, Cell Hypoxia/physiology, Cell Proliferation/physiology, Gene Expression Regulation, Neoplastic, Glioblastoma/metabolism, Humans, Hypoxia-Inducible Factor 1, alpha Subunit/metabolism, Ki-67 Antigen/metabolism, Neoplastic Stem Cells/metabolism, Nestin/metabolism, Spheroids, Cellular/metabolism, Tumor Cells, Cultured, Vascular Endothelial Growth Factor A/metabolism",

author = "Tine Rosenberg and Charlotte Aaberg-Jessen and Petterson, \{Stine Asferg\} and Kristensen, \{Bjarne Winther\}",

year = "2018",

month = apr,

doi = "10.2217/cns-2017-0034",

language = "English",

volume = "7",

pages = "CNS15",

journal = "CNS oncology",

issn = "2045-0907",

publisher = "Taylor and Francis Ltd.",

number = "2",

}

TY - JOUR

T1 - Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

AU - Rosenberg, Tine

AU - Aaberg-Jessen, Charlotte

AU - Petterson, Stine Asferg

AU - Kristensen, Bjarne Winther

PY - 2018/4

Y1 - 2018/4

N2 - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

AB - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

KW - AC133 Antigen/metabolism

KW - Antigens, Neoplasm/metabolism

KW - Biomarkers, Tumor/metabolism

KW - Brain Neoplasms/metabolism

KW - Carbonic Anhydrase IX/metabolism

KW - Cell Hypoxia/physiology

KW - Cell Proliferation/physiology

KW - Gene Expression Regulation, Neoplastic

KW - Glioblastoma/metabolism

KW - Humans

KW - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism

KW - Ki-67 Antigen/metabolism

KW - Neoplastic Stem Cells/metabolism

KW - Nestin/metabolism

KW - Spheroids, Cellular/metabolism

KW - Tumor Cells, Cultured

KW - Vascular Endothelial Growth Factor A/metabolism

UR - https://www.scopus.com/pages/publications/85059796103

U2 - 10.2217/cns-2017-0034

DO - 10.2217/cns-2017-0034

M3 - Journal article

C2 - 29708435

SN - 2045-0907

VL - 7

SP - CNS15

JO - CNS oncology

JF - CNS oncology

IS - 2

ER -

Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

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Keywords

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