Hb A2-Calderdale [δ2(NA2)His→Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report

Ida Stangerup; Jesper Petersen; Andreas Glenthøj

doi:10.1080/03630269.2021.1954945

Hb A2-Calderdale [δ2(NA2)His→Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report

Ida Stangerup, Jesper Petersen, Andreas Glenthøj

4 Citations (Scopus)

Abstract

The Hb A2-Calderdale variant [δ2(NA2)His→Asn, HBD: c.7C>A] was described as a novel variant in 2014. However, a high performance liquid chromatography (HPLC) peak was never identified indicating that this fraction could be 'hiding' somewhere else in the chromatogram. In this case report, we present evidence that the physiochemical properties of Hb A2-Calderdale resemble those of Hb A1c, resulting in a coelution in variant mode on the Tosoh G8 but not the Tosoh G11. This coelution results in an overestimation of Hb A1c and can potentially cause misdiagnosis of type 2 diabetes mellitus (T2DM).

Original language	English
Journal	Hemoglobin
Volume	45
Issue number	3
Pages (from-to)	207-209
Number of pages	3
ISSN	0363-0269
DOIs	https://doi.org/10.1080/03630269.2021.1954945
Publication status	Published - May 2021

Keywords

Hb A
Hb A-Calderdale
high performance liquid chromatography (HPLC)
δ-Globin gene

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Hb A2-Calderdale [δ2(NA2)His→Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report. / Stangerup, Ida ; Petersen, Jesper ; Glenthøj, Andreas.
In: Hemoglobin, Vol. 45, No. 3, 05.2021, p. 207-209.

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title = "Hb A2-Calderdale [δ2(NA2)His→Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report",

abstract = "The Hb A2-Calderdale variant [δ2(NA2)His→Asn, HBD: c.7C>A] was described as a novel variant in 2014. However, a high performance liquid chromatography (HPLC) peak was never identified indicating that this fraction could be 'hiding' somewhere else in the chromatogram. In this case report, we present evidence that the physiochemical properties of Hb A2-Calderdale resemble those of Hb A1c, resulting in a coelution in variant mode on the Tosoh G8 but not the Tosoh G11. This coelution results in an overestimation of Hb A1c and can potentially cause misdiagnosis of type 2 diabetes mellitus (T2DM).",

keywords = "Hb A, Hb A-Calderdale, high performance liquid chromatography (HPLC), δ-Globin gene",

author = "Ida Stangerup and Jesper Petersen and Andreas Glenth{\o}j",

year = "2021",

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doi = "10.1080/03630269.2021.1954945",

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volume = "45",

pages = "207--209",

journal = "Hemoglobin",

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TY - JOUR

T1 - Hb A2-Calderdale [δ2(NA2)His→Asn; HBD

T2 - c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report

AU - Stangerup, Ida

AU - Petersen, Jesper

AU - Glenthøj, Andreas

PY - 2021/5

Y1 - 2021/5

N2 - The Hb A2-Calderdale variant [δ2(NA2)His→Asn, HBD: c.7C>A] was described as a novel variant in 2014. However, a high performance liquid chromatography (HPLC) peak was never identified indicating that this fraction could be 'hiding' somewhere else in the chromatogram. In this case report, we present evidence that the physiochemical properties of Hb A2-Calderdale resemble those of Hb A1c, resulting in a coelution in variant mode on the Tosoh G8 but not the Tosoh G11. This coelution results in an overestimation of Hb A1c and can potentially cause misdiagnosis of type 2 diabetes mellitus (T2DM).

AB - The Hb A2-Calderdale variant [δ2(NA2)His→Asn, HBD: c.7C>A] was described as a novel variant in 2014. However, a high performance liquid chromatography (HPLC) peak was never identified indicating that this fraction could be 'hiding' somewhere else in the chromatogram. In this case report, we present evidence that the physiochemical properties of Hb A2-Calderdale resemble those of Hb A1c, resulting in a coelution in variant mode on the Tosoh G8 but not the Tosoh G11. This coelution results in an overestimation of Hb A1c and can potentially cause misdiagnosis of type 2 diabetes mellitus (T2DM).

KW - Hb A

KW - Hb A-Calderdale

KW - high performance liquid chromatography (HPLC)

KW - δ-Globin gene

UR - https://www.scopus.com/pages/publications/85111616511

U2 - 10.1080/03630269.2021.1954945

DO - 10.1080/03630269.2021.1954945

M3 - Journal article

C2 - 34309459

SN - 0363-0269

VL - 45

SP - 207

EP - 209

JO - Hemoglobin

JF - Hemoglobin

IS - 3

ER -

Hb A2-Calderdale [δ2(NA2)His→Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report

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