Gut microbiota maturity mediates the protective effect of siblings on food allergy

Yuan Gao, Jakob Stokholm, Martin O'Hely, Anne-Louise Ponsonby, Mimi L K Tang, Sarath Ranganathan, Richard Saffery, Leonard C Harrison, Fiona Collier, Lawrence Gray, David Burgner, John Molloy, Peter D Sly, Susanne Brix, Hanne Frøkiær, Peter Vuillermin

Abstract

BACKGROUND: The mechanisms underlying the protective effect of older siblings on allergic disease remain unclear but may relate to the infant gut microbiota.

OBJECTIVE: We sought to investigate whether having older siblings decreases the risk of IgE-mediated food allergy by accelerating the maturation of the infant gut microbiota.

METHODS: In a birth cohort assembled using an unselected antenatal sampling frame (n = 1074), fecal samples were collected at 1 month, 6 months, and 1 year, and food allergy status at 1 year was determined by skin prick test and in-hospital food challenge. We used 16S rRNA gene amplicon sequencing to derive amplicon sequence variants. Among a random subcohort (n = 323), microbiota-by-age z scores at each time point were calculated using fecal amplicon sequence variants to represent the gut microbiota maturation over the first year of life.

RESULTS: A greater number of siblings was associated with a higher microbiota-by-age z score at age 1 year (β  = 0.15 per an additional sibling; 95% CI, 0.05-0.24; P = .003), which was in turn associated with decreased odds of food allergy (odds ratio, 0.45; 95% CI, 0.33-0.61; P < .001). Microbiota-by-age z scores mediated 63% of the protective effect of siblings. Analogous associations were not observed at younger ages.

CONCLUSIONS: The protective effect of older siblings on the risk of developing IgE-mediated food allergy during infancy is substantially mediated by advanced maturation of the gut microbiota at age 1 year.

Original languageEnglish
JournalThe Journal of allergy and clinical immunology
Volume152
Issue number3
Pages (from-to)667-675
Number of pages9
ISSN0091-6749
DOIs
Publication statusPublished - Sept 2023

Keywords

  • Pregnancy
  • Infant
  • Humans
  • Female
  • Gastrointestinal Microbiome
  • Siblings
  • RNA, Ribosomal, 16S/genetics
  • Food Hypersensitivity/prevention & control
  • Immunoglobulin E

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