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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Growth Rate of Escherichia coli During Human Urinary Tract Infection: Implications for Antibiotic Effect

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  1. Effects of Antibiotics on the Intestinal Microbiota of Mice

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Use of a Regression Model to Study Host-Genomic Determinants of Phage Susceptibility in MRSA

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  1. Exposure of consumers to substandard antibiotics from selected authorised and unauthorised medicine sales outlets in Ghana

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  2. Oral amoxicillin and amoxicillin–clavulanic acid: properties, indications, and usage

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  3. A clear conscience is the sure sign of a bad memory: Vancomycin-resistant enterococci and rectal thermometers

    Research output: Contribution to journalLetterResearchpeer-review

  4. Effects of Antibiotics on the Intestinal Microbiota of Mice

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Antibiotic-prescribing and antibiotic-resistance patterns among elderly citizens residing in two Nordic regions

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Escherichia coli is the primary cause of urinary tract infection (UTI), which is one of the most frequent human infections. While much is understood about the virulence factors utilized by uropathogenic E. coli (UPEC), less is known about the bacterial growth dynamics taking place during infection. Bacterial growth is considered essential for successful host colonization and infection, and most antibiotics in clinical use depend on active bacterial growth to exert their effect. However, a means to measure the in situ bacterial growth rate during infection has been lacking. Due to faithful coordination between chromosome replication and cell growth and division in E. coli, chromosome replication provides a quantitative measure of the bacterial growth rate. In this study, we explored the potential for inferring in situ bacterial growth rate from a single urine sample in patients with E. coli bacteriuria by differential genome quantification (ori:ter) performed by quantitative PCR. We found active bacterial growth in almost all samples. However, this occurs with day-to-day and inter-patient variability. Our observations indicate that chromosome replication provides not only a robust measure of bacterial growth rate, but it can also be used as a means to evaluate antibiotic effect.

Original languageEnglish
JournalAntibiotics
Volume8
Issue number3
Pages (from-to)E92
ISSN2079-6382
DOIs
Publication statusPublished - 12 Jul 2019

ID: 59000533