Good performance of an immunoassay based method for nevirapine measurements in human breast milk

Kirsten Salado-Rasmussen; Zahra Persson Theilgaard; Mercy Chiduo; Court Pedersen; Jan Gerstoft; Terese Lea Katzenstein

doi:10.1515/CCLM.2011.184

Good performance of an immunoassay based method for nevirapine measurements in human breast milk

Kirsten Salado-Rasmussen, Zahra Persson Theilgaard, Mercy Chiduo, Court Pedersen, Jan Gerstoft, Terese Lea Katzenstein

4 Citations (Scopus)

Abstract

Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.

Original language	English
Journal	Clinical Chemistry and Laboratory Medicine
Volume	49
Issue number	7
Pages (from-to)	1171-5
Number of pages	5
ISSN	1434-6621
DOIs	https://doi.org/10.1515/CCLM.2011.184
Publication status	Published - 1 Jul 2011

Keywords

Calibration
Female
Humans
Immunoassay
Limit of Detection
Milk, Human
Nevirapine
Quality Control
Reproducibility of Results

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10.1515/CCLM.2011.184

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title = "Good performance of an immunoassay based method for nevirapine measurements in human breast milk",

abstract = "Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.",

keywords = "Calibration, Female, Humans, Immunoassay, Limit of Detection, Milk, Human, Nevirapine, Quality Control, Reproducibility of Results",

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AU - Salado-Rasmussen, Kirsten

AU - Persson Theilgaard, Zahra

AU - Chiduo, Mercy

AU - Pedersen, Court

AU - Gerstoft, Jan

AU - Katzenstein, Terese Lea

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AB - Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 μL) and no pre-treatment of the samples.

KW - Calibration

KW - Female

KW - Humans

KW - Immunoassay

KW - Limit of Detection

KW - Milk, Human

KW - Nevirapine

KW - Quality Control

KW - Reproducibility of Results

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JO - Clinical Chemistry and Laboratory Medicine

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Good performance of an immunoassay based method for nevirapine measurements in human breast milk

Abstract

Keywords

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