Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?

Research output: Contribution to journalJournal articleResearchpeer-review

  1. The relation of germ cells per tubule in testes, serum inhibin B and FSH in cryptorchid boys

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Is infertility after surgery for cryptorchidism congenital or acquired?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The incidence of maldescended testes in Denmark

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Newborn body composition after maternal bariatric surgery

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Laparoscopy to Assist Surgical Decisions Related to Necrotizing Enterocolitis in Preterm Neonates

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

PURPOSE: Undescended testes (UDT) are subjected to heat stress, which can disturb gonocyte transformation as well as apoptosis. This study aims to describe the apoptosis pathway occurring during minipuberty of children with unilateral (UDT), and to investigate the role of inhibin-B.

METHODS: Testicular biopsies at unilateral orchidopexy of 10 boys (6-9 months old) with normal inhibin-B (n = 5) or low inhibin-B (n = 5) were selected for immunohistochemistry and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labelling) assay. Testicular tubules were labelled with antibodies against Anti-Müllerian hormone (AMH, Sertoli cell marker), mouse Vasa Homolog (MVH) and placental alkaline phosphatase (PLAP) (both germ cell markers), cleaved caspase3 (apoptotic marker), and followed by confocal imaging and cell counting with Fiji/ImageJ. Data were analyzed with GraphPad Prism.

RESULTS: In males with low and normal inhibin-B, there was no statistical difference (p > 0.05) in the percentage of testicular tubules containing TUNEL + cells, number of cleaved caspase3 ± germ cells/tubule, total number of germ cells/tubule, and the percentage of fibrotic tubules or number of Sertoli cells/tubule.

CONCLUSIONS: These results suggest that inhibin-B does not regulate cell death of gonocytes and further studies are required to uncover any role of inhibin-B in gonocyte transformation.

Original languageEnglish
JournalPediatric Surgery International
Volume35
Issue number11
Pages (from-to)1309-1316
ISSN0179-0358
DOIs
Publication statusPublished - Nov 2019

    Research areas

  • Apoptosis, Caspase3, Cryptorchidism, Gonocyte, Inhibin-B, TUNEL

ID: 57847717